Markers of oxidative damage of nucleic acids and proteins among workers exposed to TiO2(nano) particles
| Autor: | Martin Komarc, Daniela Pelclova, Francesco Turci, Nadezda Zikova, Štěpánka Vlčková, Ingrid Corazzari, Sergey Zakharov, Zdenka Fenclova, V. Zdimal, Tomas Navratil, Petr Kacer, M Machajova, Jaroslav Schwarz, Otakar Makes, Kamila Syslová, Jaromir Belacek |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Work chemistry.chemical_element Nanoparticle 02 engineering and technology 010501 environmental sciences medicine.disease_cause 01 natural sciences Toxicology Young Adult Pigment Scanning mobility particle sizer Nucleic Acids Occupational Exposure medicine Humans Particle Size 0105 earth and related environmental sciences Titanium Chromatography technology industry and agriculture Public Health Environmental and Occupational Health Proteins Middle Aged 021001 nanoscience & nanotechnology Aerosol Oxidative Stress Breath Tests chemistry visual_art visual_art.visual_art_medium Nanoparticles Particle Particulate Matter Particle size 0210 nano-technology Oxidation-Reduction Biomarkers Oxidative stress DNA Damage |
| Zdroj: | Occupational and Environmental Medicine. 73:110-118 |
| ISSN: | 1470-7926 1351-0711 |
| DOI: | 10.1136/oemed-2015-103161 |
| Popis: | The use of nanotechnology is growing enormously and occupational physicians have an increasing interest in evaluating potential hazards and finding biomarkers of effect in workers exposed to nanoparticles.A study was carried out with 36 workers exposed to (nano)TiO2 pigment and 45 controls. Condensate (EBC) titanium and markers of oxidation of nucleic acids (including 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), 5-hydroxymethyl uracil (5-OHMeU)) and proteins (such as o-tyrosine (o-Tyr), 3-chlorotyrosine (3-ClTyr) and 3-nitrotyrosine (3-NOTyr)) were analysed from samples of their exhaled breath.In the production workshops, the median total mass 2012 and 2013 TiO2 concentrations were 0.65 and 0.40 mg/m(3), respectively. The median numbers of concentrations measured by the scanning mobility particle sizer (SMPS) and aerodynamic particle sizer (APS) were 1.98 × 10(4) and 2.32 × 10(4) particles/cm(3), respectively; and about 80% of those particles were smaller than 100 nm in diameter. In the research workspace, lower aerosol concentrations (0.16 mg/m(3) and 1.32 × 10(4) particles/cm(3)) were found. Titanium in the EBC was significantly higher in production workers (p0.001) than in research workers and unexposed controls. Accordingly, most EBC oxidative stress markers, including in the preshift samples, were higher in production workers than in the two other groups. Multiple regression analysis confirmed an association between the production of TiO2 and the levels of studied biomarkers.The concentration of titanium in EBC may serve as a direct exposure marker in workers producing TiO2 pigment; the markers of oxidative stress reflect the local biological effect of (nano)TiO2 in the respiratory tract of the exposed workers. |
| Databáze: | OpenAIRE |
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