Targetability and intracellular delivery of anti-BCG antibody-modified, pH-sensitive fusogenic immunoliposomes to tumor cells
| Autor: | Toshiro Mizoue, Motoharu Iwatsuru, Toshiya Horibe, Tomoko Takizawa, Kenji Kono, Fuminori Moriyasu, Hironobu Yanagie, Kazuo Maruyama |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Intracellular Fluid
Pathology medicine.medical_specialty Endosome medicine.drug_class media_common.quotation_subject Pharmaceutical Science Biology Endocytosis Monoclonal antibody Mice chemistry.chemical_compound Drug Delivery Systems Antigen Tumor Cells Cultured medicine Animals Internalization media_common Phosphatidylethanolamine Liposome Immunotoxins Antibodies Monoclonal Hydrogen-Ion Concentration Antibodies Bacterial Mycobacterium bovis In vitro Antibodies Anti-Idiotypic chemistry Liposomes Biophysics Cattle |
| Zdroj: | International Journal of Pharmaceutics. 237:129-137 |
| ISSN: | 0378-5173 |
| DOI: | 10.1016/s0378-5173(02)00044-3 |
| Popis: | We prepared tumor-specific immunoliposomes by coupling anti-BCG monoclonal antibodies to pH-sensitive fusogenic liposomes modified with succinylated polyglycidol (sucPG), in order to obtain efficient binding to, and endocytotic internalization into, the tumor cells. Mouse colon carcinoma 26 cells, which are known to share a common antigen with BCG, were used in in vitro experiments. BCG-sucPG immunoliposomes showed fusion ability under acidic conditions. Fluorescence microscopic observation indicated that BCG-sucPG immunoliposomes bound to colon 26 tumor cells and induced receptor-mediated endocytosis at 37 degrees C. Fusion assay by resonance energy transfer using N-(7-nitro-2-1,3-benzoxadiazol-4-yl) diacyl phosphatidylethanolamine and N-(lissamine rhodamine B sulfonyl) diacyl phosphatidylethanolamine suggested that fusion between BCG-sucPG immunoliposomes and endosomal and/or lysozomal membrane did occur. These results imply that the BCG-sucPG immunoliposomes transfer their content into the cytoplasm by fusing with the endosomal and/or lysozomal membrane after recognition of target cells and subsequent internalization into the cells by endocytosis. |
| Databáze: | OpenAIRE |
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