Autoclaving of Poloxamer 407 hydrogel and its use as a drug delivery vehicle
| Autor: | Lauren B. Priddy, Taylor Henry, Santanu Kundu, Mary Catherine Beard, Anandavalli Varadarajan, Elizabeth A. Swanson, Christine S. Grant, Leah H. Cobb |
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| Rok vydání: | 2020 |
| Předmět: |
Staphylococcus aureus
Hot Temperature Materials science Biomedical Engineering Poloxamer 02 engineering and technology complex mixtures Article Autoclave Biomaterials 03 medical and health sciences Vancomycin In vivo medicine 030304 developmental biology chemistry.chemical_classification Drug Carriers 0303 health sciences technology industry and agriculture Biomaterial Hydrogels Polymer Sterilization (microbiology) 021001 nanoscience & nanotechnology chemistry Poloxamer 407 Drug delivery Self-healing hydrogels 0210 nano-technology Biomedical engineering medicine.drug |
| Zdroj: | J Biomed Mater Res B Appl Biomater |
| ISSN: | 1552-4981 1552-4973 |
| DOI: | 10.1002/jbm.b.34703 |
| Popis: | With antibiotic-resistant bacteria becoming increasingly prevalent, biomaterials capable of targeted, in situ drug delivery are urgently needed. The synthetic polymer Poloxamer 407 (P407) is of particular interest due to its thermoreversible gelation. Clinical use of P407 typically involves sterilization via autoclaving, but the effects of these extreme environmental conditions on hydrogel water content, rheological properties and efficacy as a drug delivery vehicle remain unknown. The aim of this study was to investigate the effects of autoclaving on the properties of P407 hydrogel. Autoclaving reduced hydrogel water content due to evaporation, thus increasing the polymer weight fraction of the hydrogels. In contrast, except for a reduction in gelation temperature following autoclaving, autoclaved hydrogels had similar rheological properties as nonautoclaved hydrogels. In vitro, autoclaving did not hinder the hydrogel’s efficacy as a carrier for vancomycin antibiotic, and P407 (with and without vancomycin) had a bactericidal effect on planktonic Staphylococcus aureus. An in vivo pilot study using P407 to deliver bacteriophage highlighted the need for additional understanding of the functionality of the hydrogel for surgical applications. In conclusion, P407 hydrogel water content and gelation temperature were reduced by autoclave sterilization, while other rheological properties and the efficacy of the biomaterial as a delivery vehicle for vancomycin in vitro were unaffected. |
| Databáze: | OpenAIRE |
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