| Popis: |
Figure S7. Schematic diagram showing breeding of hybrid rice using nuclear male sterility system. WT-A & WT-B could be any female or male line, respectively, of a hybrid variety. pH-D is a CRISPR/Cas9 gene editing vector (pHUN4c12:OsDGD2β) used for targeted mutagenesis of OsDGD2β, and pC-DR is a fertility restoring vector consisting of OsDGD2β to complement the mutated gene, and a red fluorescence protein gene (RFP) for seed sorting. The transgenic seeds with pC-DR could be sorted by the red fluorescence. The male sterile, transgene-free seeds could be used in hybrid seed production by crossing with WT-B. Blue shaded box represents transgenic lines. + and – represents wild-type and mutated OsDGD2β, respectively. ⨂ represents self-pollination. Detailed information is provided in the article (Discussion 3.3). (PDF 37 kb) |
