Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach
| Autor: | Rolffy Ortiz-Andrade, Jesús Alfredo Araujo-León, Rivelino Armando Vera-Sánchez, Zulema Cantillo-Ciau, Tania Isolina Coral-Martínez, Julio Enrique Oney-Montalvo |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Naringenin Electrospray ionization Flavonoid Cmax Pharmaceutical Science QqQ 030226 pharmacology & pharmacy 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 03 medical and health sciences Hesperidin chemistry.chemical_compound 0302 clinical medicine Pharmacokinetics lcsh:Organic chemistry Limit of Detection Tandem Mass Spectrometry Drug Discovery ESI Animals flavonoid Rats Wistar Physical and Theoretical Chemistry pharmacokinetic Chromatography High Pressure Liquid Detection limit chemistry.chemical_classification validation Chromatography Chemistry 010401 analytical chemistry Organic Chemistry Selected reaction monitoring Reproducibility of Results food and beverages Rats 0104 chemical sciences ROC Curve Chemistry (miscellaneous) Area Under Curve Flavanones Molecular Medicine optimization Half-Life |
| Zdroj: | Molecules, Vol 25, Iss 4241, p 4241 (2020) Molecules Volume 25 Issue 18 |
| ISSN: | 1420-3049 |
| Popis: | The purpose of this study was to develop, optimize, and fully validate a high-sensitivity methodology using UHPLC-MS/MS to simultaneously quantify hesperidin and naringenin in microsamples (100 µ L) of murine plasma after intragastric administration of single pure flavonoids and a mixture. The optimization process allowed for high sensitivity with detection limits of approximately picogram order using an electrospray ionization (ESI) source in negative mode and an experiment based on multiple reaction monitoring (MRM). The validation parameters showed excellent linearity and detection limits, with a precision of less than 8% and a recovery of over 90%. This methodology was applied to compare the pharmacokinetic parameters for the administration of hesperidin and naringenin in individual form or in the form of a mixture. The results showed an absence of significant effects (p > 0.05) for Tmax and Cmax however, the AUC presented significant differences (p < 0.05) for both flavonoids when administered as a mixture, showing an improved absorption ratio for both flavonoids. |
| Databáze: | OpenAIRE |
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