| Autor: |
E Brown, J. P. Banga, J.M. Wicking, M.D. Sanders, E. Kasp, S Suleyman, B.A. Ellis, Dudley C. Dumonde |
| Rok vydání: |
1987 |
| Předmět: |
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| Zdroj: |
Journal of Immunological Methods. 100:147-152 |
| ISSN: |
0022-1759 |
| DOI: |
10.1016/0022-1759(87)90183-9 |
| Popis: |
This paper describes the use of phenyl-Sepharose CL-4B as a solid-phase hydrophobic adsorbent in the purification of S-antigen from protein extracts of bovine, porcine and human retina. Chromatographic conditions were ascertained whereby the majority of contaminating proteins were bound to the adsorbent leaving S-antigen in the liquid phase. In combination with size fractionation on Ultrogel AcA, the method conveniently yielded porcine and bovine S-antigen preparations up to 100% purity. Immunogenecity of purified S-antigens was verified by induction of experimental autoimmune uveoretinitis in albino Lewis rats. The method is preparative in scale, fast in performance and yields S-antigen in high purity and antigenic potency. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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