Biotechnological production, characterization and in vitro antitumor activity of polysaccharides from a native strain of Lentinus crinitus
| Autor: | Carolina Arboleda-Echavarría, Magdalena Rostro-Alanis, Juan F. Alzate, Freimar Segura-Sánchez, Xiomara López-Legarda, Roberto Parra-Saldívar, Janny A. Villa-Pulgarin |
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| Rok vydání: | 2020 |
| Předmět: |
Cell Survival
Antineoplastic Agents 02 engineering and technology Lentinula Polysaccharide Nitric Oxide Biochemistry High-performance liquid chromatography Nitric oxide Gel permeation chromatography 03 medical and health sciences chemistry.chemical_compound Mice Bioreactors Structural Biology Cell Line Tumor Bioreactor Animals Humans Molecular Biology 030304 developmental biology Cell Proliferation chemistry.chemical_classification 0303 health sciences Chemistry Tumor Necrosis Factor-alpha Macrophages Fungal Polysaccharides General Medicine Carbohydrate 021001 nanoscience & nanotechnology In vitro Fermentation MCF-7 Cells 0210 nano-technology |
| Zdroj: | International journal of biological macromolecules. 164 |
| ISSN: | 1879-0003 |
| Popis: | This work deals with the submerged cultivation, extraction and antitumor activity of polysaccharides from Lentinus crinitus. The fungus was isolated from a tropical forest (Antioquia, Colombia), cultivated in laboratory conditions, and classified by classical and molecular taxonomy. Then, it was cultivated in a bioreactor of 5 L using a ligninolytic residue as substrate. The fermentation conditions were 30 ± 1 °C, pH 4.5, 300 rpm and 1.5 vvm for 4 days. The yields of fermentation were 20 g/L of biomass. After extraction, 0.65 g/L of water-soluble exopolysaccharide (LEPS) and 3.3 mg/100 g of water-soluble intrapolysaccharide (LIPS) were obtained. In each extract total carbohydrate, glucans and protein contents were determined. Also, scanning electron microscopy (SEM), Fourier transform infrared (FTIR), X-ray diffractometry (XRD), high performance liquid chromatography with refraction index detection (HPLC-RI) and high performance gel permeation chromatography (HPGPC) analysis for characterization were performed. The antitumor activity was evaluated and polysaccharides not only showed anti-proliferative activity in breast cancer cells but also they activate J774 macrophages as evidenced by the increase of nitric oxide and tumor necrosis factor-α (inducers of tumor cell apoptosis). Our findings suggest that polysaccharides can activate macrophages to release nitric oxide (NO) and tumor necrosis factor alpha (TNF-α), which directly blocks cancer cell growth. These findings enhance our knowledge about new sources of fungal metabolites that serve as coadjuvant, cheap and less harmful alternatives to cancer treatment. |
| Databáze: | OpenAIRE |
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