Small molecule absorption by PDMS in the context of drug response bioassays

Autor: Hermanus Bernardus Johannes Karperien, Adriaan P. IJzerman, Leon G.J. Tertoolen, B.J. van Meer, Pascal Jonkheijm, Chris Denning, H. de Vries, Christine L. Mummery, J. van Weerd, Karl S.A. Firth
Přispěvatelé: Faculty of Science and Technology, Developmental BioEngineering, Molecular Nanofabrication, Applied Stem Cell Technologies
Rok vydání: 2017
Předmět:
0301 basic medicine
Microfluidics
Drug Evaluation
Preclinical
Biophysics
Context (language use)
Nanotechnology
02 engineering and technology
Biochemistry
High-performance liquid chromatography
Article
Absorption
03 medical and health sciences
chemistry.chemical_compound
Coated Materials
Biocompatible
LipoCoat Cellbinder
PDMS
Lab-On-A-Chip Devices
Materials Testing
Dimethylpolysiloxanes
Molecular Biology
Chromatography
High Pressure Liquid
chemistry.chemical_classification
Chromatography
Polydimethylsiloxane
PDMS coating
technology
industry
and agriculture
Cardiovascular Agents
Equipment Design
Cell Biology
Polymer
021001 nanoscience & nanotechnology
Lipids
Small molecule
3. Good health
Bioavailability
Equipment Failure Analysis
Nylons
030104 developmental biology
Absorption
Physicochemical
Pharmaceutical Preparations
Drug screening
chemistry
Biological Assay
Absorption (chemistry)
0210 nano-technology
Zdroj: Biochemical and Biophysical Research Communications, 482(2), 323-328. ACADEMIC PRESS INC ELSEVIER SCIENCE
Biochemical and Biophysical Research Communications
Biochemical and biophysical research communications, 482(2), 323-328. Academic Press
Biochemical and Biophysical Research Communications, 482(2), 323-328
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2016.11.062
Popis: The polymer polydimethylsiloxane (PDMS) is widely used to build microfluidic devices compatible with cell culture. Whilst convenient in manufacture, PDMS has the disadvantage that it can absorb small molecules such as drugs. In microfluidic devices like “Organs-on-Chip”, designed to examine cell behavior and test the effects of drugs, this might impact drug bioavailability. Here we developed an assay to compare the absorption of a test set of four cardiac drugs by PDMS based on measuring the residual non-absorbed compound by High Pressure Liquid Chromatography (HPLC). We showed that absorption was variable and time dependent and not determined exclusively by hydrophobicity as claimed previously. We demonstrated that two commercially available lipophilic coatings and the presence of cells affected absorption. The use of lipophilic coatings may be useful in preventing small molecule absorption by PDMS.
Graphical abstract Image 1
Highlights • Binding of different compounds to PDMS varies greatly. • Previous reported correlations of absorption and LogP values could not be repeated. • Topological polar surface area possibly related to compound absorption. • A lipid based coating partially obviates compound absorption. • Presence of cultured cells affects free drug concentration, but less than substrate.
Databáze: OpenAIRE
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