Simultaneous Determination of 18 Chemical Constituents in Traditional Chinese Medicine of Antitussive by UPLC–MS-MS
| Autor: | Qingyan Li, Li Songlin, Wang Wei, Yu Hongyan, Liu Guoru, Qiao Shi, Yu-jin Zhou, Tie-bing Liu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Thebaine Chromatography Electrospray ionization 010401 analytical chemistry Hyperoside General Medicine Mass spectrometry 01 natural sciences 0104 chemical sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Antitussive Agent medicine Liquiritigenin Liquiritin Isoliquiritigenin medicine.drug |
| Zdroj: | Journal of Chromatographic Science. 54:1540-1552 |
| ISSN: | 1945-239X 0021-9665 |
| DOI: | 10.1093/chromsci/bmw099 |
| Popis: | In this study, a simple, rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed for the simultaneous determination of 18 compounds, namely, morphine, codeine, tuberostemonine, thebaine, papaverine, scopoletin, liquiritin, narcotine, gynaroside, hyperoside, hesperidin, isoliquiritin, liquiritigenin, luteolin, isoliquiritigenin, apigenin, formononetin and glycyrrhizic acid in traditional Chinese medicine of antitussive. Analytes were chromatographically separated on a Waters C18 column under gradient elution with a mobile phase of water containing 0.1‰ HOAc (A) and methanol (MeOH) containing 0.1‰ HOAc (B). Detection was accomplished by multiple-reaction-monitoring scanning with an electrospray ionization source under positive and negative ion switching modes. Samples were prepared with dilution and ultrasonic extraction. All components were detected within the linear regime (r2 ≥ 0.9924) at the concentration ranges tested. The precision ranged from 1.3 to 4.6%, the accuracy was between 85.6 and 116.7% and the results of method validation tests were all within the required limits. The validated quantification method was successfully applied to analyze 40 batches of commercial traditional Chinese medicine of antitussive. These results will provide a basis for quality control of production process and the further pharmacokinetic study of traditional Chinese medicine of antitussive in vivo. |
| Databáze: | OpenAIRE |
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