Cationic lipid-assisted nanoparticles for delivery of mRNA cancer vaccine
| Autor: | Song Shen, Ying-Li Luo, Qian Chen, Zhen Gu, Li Wang, Hou-Bing Zhang, Jun Wang, Ya-Nan Fan, Min Li |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Lymphoma Biomedical Engineering 02 engineering and technology CD8-Positive T-Lymphocytes Cancer Vaccines Cell Line Mice 03 medical and health sciences Immune system Antigen Animals Cytotoxic T cell General Materials Science RNA Messenger Drug Carriers Messenger RNA biology Chemistry Dendritic Cells 021001 nanoscience & nanotechnology Lipids In vitro Cell biology Mice Inbred C57BL Ovalbumin 030104 developmental biology Cell culture biology.protein Nanoparticles Cancer vaccine 0210 nano-technology |
| Zdroj: | Biomaterials Science. 6:3009-3018 |
| ISSN: | 2047-4849 2047-4830 |
| DOI: | 10.1039/c8bm00908b |
| Popis: | Message RNA-based vaccines with prominent advantages such as facile production, no requirement for nuclear entry and high safety without the need for integration into host genome have been shown to be potent activators of the cytotoxic immune system. However, wider applications of mRNA-based therapeutics have been hindered because of their intrinsically high vulnerability to expressed nucleases and difficulty while entering antigen-presenting cells (APCs) directly. Here, we investigated the potential of cationic lipid-assisted nanoparticles (CLAN), which form a clinically translatable nucleic acid delivery system working as a carrier of an mRNA vaccine. We found that CLAN encapsulating mRNA encoding antigen could effectively stimulate the maturation of dendritic cells (DCs) and promote the activation and proliferation of antigen-specific T cells both in vitro and in vivo. Intravenous immunization of mice with CLAN containing mRNA encoding ovalbumin (OVA) provoked a strong OVA-specific T-cell response and slowed tumor growth in an aggressive E·G7-OVA lymphoma model. Collectively, CLAN proved to be a promising platform for mRNA vaccine delivery. |
| Databáze: | OpenAIRE |
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