Structure and expression of the ornithine decarboxylase gene of Chlamydomonas reinhardtii
| Autor: | Martina Fausel, Karl-Heinz Adam, Otfried Marquardt, Jürgen Voigt, Peter Bohley |
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| Rok vydání: | 2005 |
| Předmět: |
DNA
Complementary genetic structures Molecular Sequence Data Chlamydomonas reinhardtii Biology Ornithine Decarboxylase Microbiology Ornithine decarboxylase Open Reading Frames DNA Algal Complementary DNA Gene expression Animals Amino Acid Sequence RNA Messenger Cloning Molecular Peptide sequence Gene Ornithine decarboxylase antizyme Phylogeny Base Sequence Sequence Homology Amino Acid fungi Chlamydomonas Sequence Analysis DNA biology.organism_classification RNA Algal Biochemistry |
| Zdroj: | Microbiological research. 159(4) |
| ISSN: | 0944-5013 |
| Popis: | A cDNA was cloned encoding ornithine decarboxylase (ODC) of the unicellular green alga Chlamydomonas reinhardtii. The polypeptide consists of 396 amino acid residues with 35-37% sequence identity to other eukaryotic ODCs. As indicated by the phylogenetic tree calculated by neighbour joining analysis, the Chlamydomonas ODC has the same evolutionary distances to the ODCs of higher plants and mammalians. The Chlamydomonas ODC gene contains three introns of 222, 133, and 129bp, respectively. As revealed by Northern-blot analyses, expression of the Chlamydomonas ODC gene is neither altered throughout the vegetative cell cycle nor modulated by exogenous polyamines. |
| Databáze: | OpenAIRE |
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