Baseline analysis of Mycoplasma mycoides subsp. mycoides antigens as targets for a DIVA assay for use with a subunit vaccine for contagious bovine pleuropneumonia

Autor:	Emil M. Berberov, Harrison O. Lutta, Roger Pelle, Arshad Mather, David Odongo, Moses Olum, Jose Perez-Casal, Tracy Prysliak, Volker Gerdts, Andrew A. Potter, Jan Naessens, Alexander Kipronoh, Martina Kyallo
Rok vydání:	2020
Předmět:	Male Cattle Diseases Enzyme-Linked Immunosorbent Assay DIVA medicine.disease_cause law.invention 03 medical and health sciences Contagious bovine pleuropneumonia Mycoplasma Antigen law medicine Animals CBPP Antigens Pleuropneumonia Contagious 030304 developmental biology 0303 health sciences Antigens Bacterial lcsh:Veterinary medicine General Veterinary biology 030306 microbiology Mycoplasma mycoides General Medicine biology.organism_classification medicine.disease Virology Antibodies Bacterial Diva Bacterial vaccine Bacterial Vaccines Vaccines Subunit Recombinant DNA biology.protein lcsh:SF600-1100 Cattle Antibody Research Article
Zdroj:	BMC Veterinary Research BMC Veterinary Research, Vol 16, Iss 1, Pp 1-12 (2020)
ISSN:	1746-6148
Popis:	Background Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia in cattle. A prototype subunit vaccine is being developed, however, there is currently no diagnostic test that can differentiate between infected cattle and those vaccinated with the prototype subunit vaccine. This study characterized Mmm proteins to identify potential antigens for use in differentiating infected from vaccinated animals. Results Ten Mmm antigens expressed as recombinant proteins were tested in an indirect ELISA using experimental sera from control groups, infected, and vaccinated animals. Data were imported into R software for analysis and drawing of the box and scatter plots while Cohen’s Kappa assessed the level of agreement between the Mmm antigens. Two vaccine antigens (MSC_0499 and MSC_0776) were superior in detecting antibodies in sera of animals vaccinated with the subunit vaccines while two non-vaccine antigens (MSC_0636 and LppB) detected antibodies in sera of infected animals showing all clinical stages of the disease. Sensitivity and specificity of above 87.5% were achieved when the MSC_0499 and MSC_0636 antigens were tested on sera from vaccinated and infected animals. Conclusions The MSC_0499 and MSC_0776 antigens were the most promising for detecting vaccinated animals, while MSC_0636 and LppB were the best targets to identify infected animals. Further testing of sera from vaccinated and infected animals collected at different time intervals in the field should help establish how useful a diagnostic test based on a cocktail of these proteins would be.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e70a9fdc1c513ae149c7d68517c6132d https://pubmed.ncbi.nlm.nih.gov/32650780 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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