Progression of microalbuminuria in SHR is associated with lower expression of critical components of the apical endocytic machinery in the renal proximal tubule
| Autor: | Ana Luiza T. Barreto, Adriana C. C. Girardi, Mariliza V. Rodrigues, Daniel F. Arruda-Junior, José Eduardo Krieger, Bruna Hitomi Inoue, Luciene Cristina Gastalho Campos |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Physiology Endocytic cycle Blood Pressure Receptors Cell Surface Disease urologic and male genital diseases Essential hypertension Renal protein reabsorption Kidney Tubules Proximal Rats Inbred SHR Internal medicine medicine Albuminuria Animals Rats Wistar business.industry Transferrin medicine.disease Cubilin Endocytosis Rats Peripheral Low Density Lipoprotein Receptor-Related Protein-2 medicine.anatomical_structure Endocrinology Hypertension Disease Progression Proximal tubule Microalbuminuria business |
| Zdroj: | American Journal of Physiology-Renal Physiology. 305:F216-F226 |
| ISSN: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.00255.2012 |
| Popis: | Cumulative epidemiological evidence indicates that the presence of microalbuminuria predicts a higher frequency of cardiovascular events, peripheral disease, and mortality in essential hypertension. Microalbuminuria may arise from increased glomerular permeability and/or reduced proximal tubular reabsorption of albumin by receptor-mediated endocytosis. This study aimed to evaluate the temporal pattern of urinary protein excretion and to test the hypothesis that progression of microalbuminuria is associated with decreased protein expression of critical components of the endocytic apparatus in the renal proximal tubule of spontaneously hypertensive rats (SHR). We found that urinary albumin excretion increased progressively with blood pressure in SHR from 6 to 21 wk of age. In addition, SDS-PAGE analysis of urinary proteins showed that microalbuminuric SHR virtually excreted proteins of the size of albumin or smaller (+-ATPase B2 subunit was also reduced in the renal cortex of 21-wk-old compared with both 6- and 14-wk-old SHR. Collectively, our study suggests that enhanced urinary protein excretion, especially of albumin, may be due, at least in part, to lower expression of key components of the apical endocytic apparatus in the renal proximal tubule. Finally, one may speculate that dysfunction of the apical endocytic pathway in the renal proximal tubule may contribute to the development of microalbuminuria in essential hypertension. |
| Databáze: | OpenAIRE |
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