Demonstration of human Borna disease virus RNA in human peripheral blood mononuclear cells
| Autor: | Kazuyoshi Ikuta, Masanori Senjo, Yurie Nakamura, Takaaki Nakaya, Kazuhiko Ikeda, Qiu Zhong, Mitsuaki Kakinuma, Masahiko Kishi, Shiro Kato |
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| Rok vydání: | 1995 |
| Předmět: |
Blood transfusion
medicine.medical_treatment animal diseases viruses Molecular Sequence Data Biophysics RT-PCR Disease Antibodies Viral Biochemistry Peripheral blood mononuclear cell Polymerase Chain Reaction Virus law.invention Pathogenesis Japan Structural Biology law Ethidium Genetics medicine Humans Borna disease virus Molecular Biology Polymerase chain reaction Repetitive Sequences Nucleic Acid Borna disease biology Base Sequence Staining and Labeling Mental Disorders virus diseases RNA-Directed DNA Polymerase Cell Biology Virology Blotting Southern Immunology biology.protein Leukocytes Mononuclear RNA Viral Antibody |
| Zdroj: | FEBS letters. 364(3) |
| ISSN: | 0014-5793 |
| Popis: | BDV naturally infects horses and sheep, and causes sporadic neurological disease. Serological evidence suggests an association of BDV, or a related virus, with specific psychiatric diseases in humans. Here, by using a nested RT-PCR technique, we demonstrate that human BDV RNA is present in the PBMC of psychiatric patients. In an examination of a total of 60 patients from 5 wards of a hospital in Japan, the detection rate differed within each ward, ranging from 8% to > 50% (37% on the average). Of particular note was the finding that the human derived BDV sequences, which included deleted forms in about 23% of the positive samples, were slightly different from those derived from horse BDV. These results suggest urgent consideration of the measures to be taken to cope with the effects of blood transfusion. In addition, the detection of a high level of BDV in the PBMC of patients will help our understanding of the pathogenesis in the disease. |
| Databáze: | OpenAIRE |
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