Tissue‐specific expression of ribosomal protein paralogueeRpL22 ‐like inDrosophila melanogastereye development
| Autor: | Caroline E. Pritchard, Kenneth Chaney, Vassie C. Ware, Brett W. Gershman |
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| Rok vydání: | 2020 |
| Předmět: |
Ribosomal Proteins
0301 basic medicine Cell type Embryo Nonmammalian Biology Eye Ribosome Article 03 medical and health sciences 0302 clinical medicine Ribosomal protein Polysome Gene expression Animals Drosophila Proteins Gene Expression Regulation Developmental biology.organism_classification Phenotype Cell biology Drosophila melanogaster 030104 developmental biology Organ Specificity Eye development 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Dev Dyn |
| ISSN: | 1097-0177 1058-8388 |
| DOI: | 10.1002/dvdy.185 |
| Popis: | Background Differences in core or tissue-specific ribosomal protein (Rp) composition within ribosomes contribute to ribosome heterogeneity and functional variability. Yet, the degree to which ribosome heterogeneity modulates development is unknown. The Drosophila melanogaster eRpL22 family contains structurally diverse paralogues, eRpL22 and eRpL22-like. Unlike ubiquitously expressed eRpL22, eRpL22-like expression is tissue-specific, notably within the male germline and the eye. We investigated expression within the developing eye to uncover tissue/cell types where specific paralogue roles might be defined. Results Immunohistochemistry analysis confirms ubiquitous eRpL22 expression throughout eye development. In larvae, eRpL22-like is ubiquitously expressed, but highly enriched in the peripodial epithelium (PE). In early pupae, eRpL22-like is broadly distributed in multiple cell types, but later, is primarily enriched in interommatidial hair cells (IoHC). Adult patterns include the ring of accessory cells around ommatidia. Adult retinae IoHC patterning phenotypes (shown by scanning electron microscopy) may be linked to RNAi-mediated eRpL22-like depletion within larval PE. Immunoblots and polysome profile analyses show multiple variants of eRpL22-like across development, with the variant at the expected molecular mass co-sedimenting with active ribosomes. Conclusion Our data reveal differential patterns of eRpL22-like expression relative to eRpL22 and suggest a specific role for eRpL22-like in developmental patterning of the eye. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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