Dysfunctional protection against advanced glycation due to thiamine metabolism abnormalities in gestational diabetes
Autor: | Katarína Kuricová, Anna Pleskačová, Lukáš Pácal, Veronika Dvořáková, Marie Tomandlová, Josef Tomandl, Vendula Bartáková, Jana Bělobrádková, Kateřina Kaňková |
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Rok vydání: | 2016 |
Předmět: |
Adult
Glycation End Products Advanced medicine.medical_specialty Erythrocytes 030209 endocrinology & metabolism Type 2 diabetes 030204 cardiovascular system & hematology Transketolase Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pregnancy Internal medicine medicine Humans Molecular Biology biology Chemistry Thiamine transport Membrane Transport Proteins nutritional and metabolic diseases food and beverages Cell Biology medicine.disease Gestational diabetes Diabetes Gestational Endocrinology SLC19A3 biology.protein SLC19A2 Female Thiamine Thiamine Pyrophosphate Thiamine pyrophosphate Follow-Up Studies |
Zdroj: | Glycoconjugate Journal. 33:591-598 |
ISSN: | 1573-4986 0282-0080 |
Popis: | While the pathogenic role of dicarbonyl stress and accelerated formation of advanced glycation end products (AGEs) to glucose intolerance and to the development of diabetic complications is well established, little is known about these processes in gestational diabetes mellitus (GDM), a condition pathogenically quite similar to type 2 diabetes. The aims of the present study were (i) to determine plasma thiamine and erythrocyte thiamine diphosphate (TDP) and transketolase (TKT) activity in pregnant women with and without GDM, (ii) to assess relationships between thiamine metabolism parameters and selected clinical, biochemical and anthropometric characteristics and, finally, (iii) to analyse relationship between variability in the genes involved in the regulation of transmembrane thiamine transport (i.e. SLC19A2 and SLC19A3) and relevant parameters of thiamine metabolism. We found significantly lower plasma BMI adjusted thiamine in women with GDM (P = 0.002, Mann-Whitney) while levels of erythrocyte TDP (an active TKT cofactor) in mid-trimester were significantly higher in GDM compared to controls (P = 0.04, Mann-Whitney). However, mid-gestational TKT activity - reflecting pentose phosphate pathway activity - did not differ between the two groups (P > 0.05, Mann-Whitney). Furthermore, we ascertained significant associations of postpartum TKT activity with SNPs SLC19A2 rs6656822 and SLC19A3 rs7567984 (P = 0.03 and P = 0.007, resp., Kruskal-Wallis). Our findings of increased thiamine delivery to the cells without concomitant increase of TKT activity in women with GDM therefore indicate possible pathogenic role of thiamine mishandling in GDM. Further studies are needed to determine its contribution to maternal and/or neonatal morbidity. |
Databáze: | OpenAIRE |
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