Atmospheric Pressure Drift Tube Ion Mobility–Orbitrap Mass Spectrometry: Initial Performance Characterization
| Autor: | Joel D. Keelor, Anyin Li, Brian H. Clowers, Stephen Zambrzycki, Facundo M. Fernández |
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| Rok vydání: | 2017 |
| Předmět: |
Chromatography
Chemistry Ion-mobility spectrometry 010401 analytical chemistry Selected reaction monitoring Analytical chemistry 010402 general chemistry Orbitrap Top-down proteomics Tandem mass spectrometry Mass spectrometry 01 natural sciences 0104 chemical sciences Analytical Chemistry law.invention Ion-mobility spectrometry–mass spectrometry law Hybrid mass spectrometer |
| Zdroj: | Analytical Chemistry. 89:11301-11309 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.7b01866 |
| Popis: | Atmospheric pressure drift tube ion mobility spectrometry (AP-DTIMS) was coupled with Fourier transform Orbitrap mass spectrometry. The performance capabilities of this versatile new arrangement were demonstrated for different DTIMS ion gating operation modes and Orbitrap mass spectrometer parameters with regard to sensitivity and resolving power. Showcasing the optimized AP-DTIMS-Orbitrap MS system, isobaric peptide and sugar isomers were successfully resolved and the identities of separated species validated by high-energy collision dissociation experiments. |
| Databáze: | OpenAIRE |
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