In vivo assay for somatic point mutations induced by genotoxic carcinogens: incorporation of [35S]methionine into a rat liver cytochrome b5 normally lacking sulphur-containing amino acids
| Autor: | Werner K. Lutz, Arthur Jauch |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Male
Nitrosamines Biology Toxicology Sulfur Radioisotopes chemistry.chemical_compound Methionine Toxikologie In vivo Cytochrome b5 medicine Animals Diethylnitrosamine Trypsin ddc:610 Carcinogen chemistry.chemical_classification Mutagenicity Tests Rats Inbred Strains General Medicine Cytochrome b Group Molecular biology Peptide Fragments Amino acid Rats Kinetics Cytochromes b5 chemistry Biochemistry Mutation Nucleic acid Carcinogens Microsomes Liver Cysteine medicine.drug Mutagens |
| Zdroj: | Chemico-biological interactions. 46(2) |
| ISSN: | 0009-2797 |
| Popis: | The trypsin fragments of rat liver microsomal cytochron1e b\(_5\) (Tb\(_5\)) lack both methionine (met) and cysteine (cys), i.e., the sulphur-containing antino acids. Tb\(_5\) should therefore contain no 358-radioactivity after isolation from animals treated wHh [\(^{35}\)S]met or [\(^{36}\)S]cys. If, however, the nucleic acids coding for this polypeptide have been damaged by a genotoxic carcinogen, a miscoding could result in an incorporation of met or cys into the polypeptide so that Tb\(_8\) could now be \(^{36}\)S-radiolabelled. Two experiments are descrihed. the first one where a toxic regimen of N -nitrosomorpholine (NNM) to rats resulted in a significant increase of \(^{35}\)S-radioactivity in the Tbs of liver microsomes, and a second experiment with a non-toxic regimen of N,N diethylnitrosamine (DENA), where no increase was observable. |
| Databáze: | OpenAIRE |
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