Functional importance of connective tissue repair during the development of experimental abdominal aortic aneurysms
| Autor: | Gail Moore, D.Brent Kerns, Mark D. Huffman, Robert W. Thompson, Barry C. Starcher, John A. Curci |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
medicine.medical_specialty Pathology Swine Urology Connective tissue Desmosine Protein-Lysine 6-Oxidase Aneurysm Tropoelastin medicine.artery medicine Animals Aorta Abdominal Rats Wistar Pancreatic elastase Aortic dissection Aorta Wound Healing biology Pancreatic Elastase business.industry Reverse Transcriptase Polymerase Chain Reaction Elastase Abdominal aorta medicine.disease Rats Hydroxyproline medicine.anatomical_structure Connective Tissue Aminopropionitrile cardiovascular system biology.protein Surgery business Elastin Procollagen Aortic Aneurysm Abdominal |
| Zdroj: | Surgery. 128(3) |
| ISSN: | 0039-6060 |
| Popis: | Background. Abdominal aortic aneurysms (AAAs) involve an unfavorable balance between the destruction and the repair of connective tissue proteins. The purpose of this study was to assess the functional importance of connective tissue repair during experimental aneurysmal degeneration. Methods. Male Wistar rats (n = 70) underwent transient intraluminal perfusion of the abdominal aorta with porcine pancreatic elastase. In Study I, the aortic diameter was measured before elastase perfusion and at days 0, 2, 7, and 14 (n = 6 rats at each interval). Aortic wall concentrations of desmosine (Des) and hydroxyproline (OHP) were measured at each interval, and the expression of tropoelastin (TE), α1(I) procollagen (PC), and lysyl oxidase genes was evaluated by reverse transcription-polymerase chain reaction. In Study II, 22 rats were treated with β-aminopropionitrile (BAPN) to block connective tissue repair. In Study III (n = 30), rats were treated with doxycycline, a matrix metalloproteinase inhibitor, beginning 7 days after elastase perfusion. Results. AAAs consistently developed between 7 and 14 days after elastase perfusion. Aortic wall Des concentration decreased markedly during aneurysm development, reaching 3% of normal by day 14 (377 ± 22 pmol of Des/sample on day 0 vs 9 ± 1 pmol of Des/sample on day 14; P |
| Databáze: | OpenAIRE |
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