Oral biofilm models for mechanical plaque removal
| Autor: | Frank Abbas, Minie Rustema-Abbing, Martinus J. Verkaik, Henk J. Busscher, Anje M. Slomp, Henny C. van der Mei |
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| Přispěvatelé: | Man, Biomaterials and Microbes (MBM), Personalized Healthcare Technology (PHT) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
Dental plaque Toothbrushing Saliva GINGIVAL ABRASION Dental pellicle Sonic brush ADHESION Models Biological Bacterial Adhesion SONIC TOOTHBRUSH Microbiology Streptococcus mutans Sonication medicine Actinomyces Humans Dental Pellicle General Dentistry Analysis of Variance biology ACTINOMYCES-NAESLUNDII Chemistry Dentistry(all) Non-contact removal Biofilm Streptococcus oralis SALIVARY GLYCOPROTEINS IN-VITRO biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease EFFICACY STREPTOCOCCUS-MUTANS stomatognathic diseases REDUCTION Biofilms BACTERIA Actinomyces naeslundii Female Original Article |
| Zdroj: | Clinical Oral Investigations, 14(4), 403-409. SPRINGER HEIDELBERG Clinical Oral Investigations |
| ISSN: | 1436-3771 1432-6981 |
| Popis: | In vitro plaque removal studies require biofilm models that resemble in vivo dental plaque. Here, we compare contact and non-contact removal of single and dual-species biofilms as well as of biofilms grown from human whole saliva in vitro using different biofilm models. Bacteria were adhered to a salivary pellicle for 2 h or grown after adhesion for 16 h, after which, their removal was evaluated. In a contact mode, no differences were observed between the manual, rotating, or sonic brushing; and removal was on average 39%, 84%, and 95% for Streptococcus mutans, Streptococcus oralis, and Actinomyces naeslundii, respectively, and 90% and 54% for the dual- and multi-species biofilms, respectively. However, in a non-contact mode, rotating and sonic brushes still removed considerable numbers of bacteria (24-40%), while the manual brush as a control (5-11%) did not. Single A. naeslundii and dual-species (A. naeslundii and S. oralis) biofilms were more difficult to remove after 16 h growth than after 2 h adhesion (on average, 62% and 93% for 16- and 2-h-old biofilms, respectively), while in contrast, biofilms grown from whole saliva were easier to remove (97% after 16 h and 54% after 2 h of growth). Considering the strong adhesion of dual-species biofilms and their easier more reproducible growth compared with biofilms grown from whole saliva, dual-species biofilms of A. naeslundii and S. oralis are suggested to be preferred for use in mechanical plaque removal studies in vitro. |
| Databáze: | OpenAIRE |
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