Decreased Expression of α2,8 Sialyltransferase and Increased Expression of β1,4 N-Acetylgalactosaminyltransferase in Gastrointestinal Cancers
| Autor: | Hisao Fukumoto, Kazuto Nishio, Takashi Nakamura, Makoto Iwahashi, Yasuaki Tatsumi, Yasuhiro Koh, Hiroshi Tanimura, Mikiko Shimizu, Takuya Tsunoda, Nagahiro Saijo, Kiwao Ishimoto, Hiroki Yamaue |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male 0301 basic medicine Pathology medicine.medical_specialty Sialyltransferase Colorectal cancer Down-Regulation Gene Expression Regulation Enzymologic General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Sex Factors 0302 clinical medicine Stomach Neoplasms Gangliosides medicine Humans Gastrointestinal cancer Stomach cancer Lung cancer beta-D-Galactoside alpha 2-6-Sialyltransferase Aged Ganglioside biology Middle Aged medicine.disease Sialyltransferases Gene Expression Regulation Neoplastic GD2 Antibody 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Cancer research N-Acetylgalactosaminyltransferases Female lipids (amino acids peptides and proteins) Antibody Colorectal Neoplasms |
| Zdroj: | Experimental Biology and Medicine. 227:196-200 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.1177/153537020222700307 |
| Popis: | Gangliosides such as GD3, GM2, and GD2 are abundantly expressed on the cell surfaces of various malignant cells, suggesting the potential for anti-ganglioside antibody therapy for tumors. Anti-ganglioside GD2 antibody treatment is currently undergoing clinical trials for melanoma and neuroblastoma. We previously reported high in vivo antitumor effects of anti-GM2 ganglioside antibody against lung cancer. To determine whether anti-GM2 antibody may be clinically indicated for gastrointestinal cancers, we evaluated the mRNA expression of α2,8 sialyltransferase, a GD3 synthase, and β1,4 N-acetylgalactosaminyltransferase (β1,4 GalNAc-T), a GM2/GD2 synthase, in gastrointestinal cancers. We performed modified semi-quantitative RT-PCR, which reduces complexity incidental to radiolabeling on samples taken from small surgically removed clinical specimens. Stomach (19/22) and colorectal (21/30) cancers showed decreased expression of α2,8 sialyltransferase as compared with respective normal tissues (P < 0.05). In contrast, increased expression of β1,4 GalNAc-T was detected in both types of tumors. Clinicopathological analysis revealed significantly higher expression level of α2,8 sialyltransferase in the poorly differentiated than in the well-differentiated stomach cancer group (P < 0.05). Furthermore, the expression level of α2,8 sialyltransferase was significantly decreased in male as compared with female colorectal cancer patients (P < 0.05). These results suggest that expression level of GM2 ganglioside is elevated in gastrointestinal cancer, and that anti-GM2 antibody may be applicable to its treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|