Common variation in the C-terminal region of the fibrinogen β-chain: effects on fibrin structure, fibrinolysis and clot rigidity
| Autor: | Robert A. S. Ariëns, D. Alastair Smith, Robert Harrand, John W. Weisel, Ramzi A. Ajjan, Fladia Phoenix, Sarah Dolling, Kristina F. Standeven, Simon D. Connell, Bernard C. B. Lim, Peter J. Grant, Richard B. Greaves, Radwa H. Abou-Saleh |
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| Rok vydání: | 2008 |
| Předmět: |
Models
Molecular Magnetic tweezers Lysis medicine.medical_treatment Immunology Coronary Artery Disease Fibrinogen Biochemistry Fibrin law.invention Protein structure law Chlorocebus aethiops Fibrinolysis medicine Animals Humans Polymorphism Genetic COS cells biology Cell Biology Hematology Recombinant Proteins Protein Structure Tertiary Stroke Amino Acid Substitution COS Cells Biophysics biology.protein Recombinant DNA medicine.drug |
| Zdroj: | Blood. 111:643-650 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2007-05-091231 |
| Popis: | Fibrinogen BβArg448Lys is a common polymorphism, positioned within the carboxyl terminus of the Bβ-chain of the molecule. Studies suggest that it is associated with severity of coronary artery disease and development of stroke. The effects of the amino acid substitution on clot structure remains controversial, and the aim of this study was to investigate the effect(s) of this polymorphism on fibrin clot structure using recombinant techniques. Permeation, turbidity, and scanning electron microscopy showed that recombinant Lys448 fibrin had a significantly more compact structure, with thin fibers and small pores, compared with Arg448. Clot stiffness, measured by means of a novel method using magnetic tweezers, was significantly higher for the Lys448 compared with the Arg448 variant. Clots made from recombinant protein variants had similar lysis rates outside the plasma environment, but when added to fibrinogen-depleted plasma, the fibrinolysis rates for Lys448 were significantly slower compared with Arg448. This study demonstrates for the first time that clots made from recombinant BβLys448 fibrinogen are characterized by thin fibers and small pores, show increased stiffness, and appear more resistant to fibrinolysis. Fibrinogen BβArg448Lys is a primary example of common genetic variation with a significant phenotypic effect at the molecular level. |
| Databáze: | OpenAIRE |
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