Immunodiagnosis of Trichinella infection in the horse
| Autor: | Ljiljana Sofronic-Milosavljevic, Edoardo Pozio, H.R. Gamble, M. A. Gomez Morales, I.V. Patrascu, N. Skerovic |
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| Rok vydání: | 2001 |
| Předmět: |
Veterinary (miscellaneous)
Blotting Western Prevalence Antibodies Helminth Trichinella serology Enzyme-Linked Immunosorbent Assay Trichinosis Biology Serology lcsh:Infectious and parasitic diseases Western blot Antigen medicine Animals lcsh:RC109-216 Horses medicine.diagnostic_test Romania Horse Trichinellosis medicine.disease biology.organism_classification Virology horse Infectious Diseases Insect Science Antigens Helminth biology.protein Animal Science and Zoology Parasitology epidemiology Horse Diseases Protein A |
| Zdroj: | Parasite, Vol 8, Pp S260-S262 (2001) |
| ISSN: | 1252-607X |
| Popis: | From 1998 to 2000, 5,267 horse sera were collected from several Trichinella regions in Romania. Sera were initially screened in laboratories in Romania, Serbia and Italy with an ELISA and a Western blot (Wb) using an excretory/secretory (ES) antigen and several conjugates (protein A, protein G, and sheep or goat anti-horse). Differences in serology results were obtained among the different conjugates and also between ELISA and Wb. Depending on the test used, specific antibodies were found at a prevalence rate of 3-6 % of horses. Serum samples classified as positive were tested again by ELISA using a synthetic tyvelose glycan-BSA antigen, in Italy. All serum samples tested using this antigen were negative; in contrast, serum samples from experimentally infected horses were positive with the glycan antigen. The negative results obtained with the glycan antigen are consistent with the low prevalence of horse trichinellosis reported in the literature. Based on these results, further studies are needed to validate immunodiagnostic tests to detect Trichinella infection in horses. |
| Databáze: | OpenAIRE |
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