Increased Expression of Musashi-1 Evidences Mesenchymal Repair in Maxillary Sinus Floor Elevation
| Autor: | Francisco O'Valle, Juan G. de Buitrago, Vicente Crespo-Lora, D. Aguilar, Miguel Padial-Molina, Pablo Galindo-Moreno, Marién Cobo, Pedro Hernández-Cortés |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Sinus Floor Augmentation Pathology medicine.medical_specialty Maxillary sinus Biopsy Cellular differentiation lcsh:Medicine Nerve Tissue Proteins Osteocytes Article 03 medical and health sciences 0302 clinical medicine Smooth muscle Humans Medicine RNA Messenger lcsh:Science Aged Osteoblasts Multidisciplinary medicine.diagnostic_test business.industry Regeneration (biology) lcsh:R Mesenchymal stem cell RNA-Binding Proteins Cell Differentiation Mesenchymal Stem Cells 030206 dentistry Maxillary Sinus Middle Aged 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Immunohistochemistry Female lcsh:Q business |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-9 (2018) Digibug. Repositorio Institucional de la Universidad de Granada instname Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | This study aimed to analyze the expression of Musashi-1 (MSI1) in maxillary native bone and grafted bone after maxillary sinus floor elevation. To do so, fifty-seven bone biopsies from 45 participants were studied. Eighteen samples were collected from native bone while 39 were obtained 6 months after maxillary sinus grafting procedures. Musashi-1 was analyzed by immunohistochemistry and RT-PCR. MSI1 was detected in osteoblasts and osteocytes in 97.4% (38/39) of grafted areas. In native bone, MSI1 was detected in only 66.6% (12/18) of the biopsies, mainly in osteocytes. Detection of MSI1 was significantly higher in osteoprogenitor mesenchymal cells of grafted biopsies (p < 0.001) but minor in smooth muscle and endothelial cells; no expression was detected in adipocytes. The mesenchymal cells of the non-mineralized tissue of native bone showed very low nuclear expression of MSI1, in comparison to fusiform cells in grafted areas (0.28(0.13) vs. 2.10(0.14), respectively; p < 0.001). Additionally, the detection of MSI1 mRNA was significantly higher in biopsies from grafted areas than those from native bone (1.00(0.51) vs. 60.34(35.2), respectively; p = 0.029). Thus, our results regardig the significantly higher detection of Musashi-1 in grafted sites than in native bone reflects its importance in the remodeling/repair events that occur after maxillary sinus floor elevation in humans. This investigation was partially supported by Research Groups #CTS-138 and #CTS-1028 (Junta de Andalucía, Spain). MPM was supported by the Andalucía Talent Hub Program from the Andalusian Knowledge Agency (co-funded by the European Union’s Seventh Framework Program, Marie Skłodowska-Curie actions (COFUND – Grant Agreement n° 291780) and the Ministry of Economy, Innovation, Science and Employment of the Junta de Andalucía). |
| Databáze: | OpenAIRE |
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