Characterization of a Novel Porcine CSN2 Polymorphism and Its Distribution in Five European Breeds

Autor: Augustin Vlaic, Mihai Șuteu, S. Dărăban
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Animals, Vol 9, Iss 7, p 419 (2019)
Animals
Volume 9
Issue 7
Animals : an Open Access Journal from MDPI
ISSN: 2076-2615
Popis: Here, we describe a novel porcine &beta
casein (CNS2) polymorphism, initially identified using the isoelectric focusing (IEF) technique, and provide its distribution in five European breeds. Porcine CSN2 cDNA samples, from sows identified using IEF as carriers of polymorphic variants, were sequenced, and based on the sequence alignments, a genotyping assay was developed. The distribution of the polymorphism was investigated by genotyping 167 sows. Population genetic indexes were computed using POPGENE32 version 1.32. Sequence alignments revealed that the mutation which caused the different &beta
casein IEF migration profiles was c.647G>
A, a substitution located in exon 7, which modifies the amino acid from position 201 of the mature protein from arginine to glutamine. The frequency of the G allele was 0.965 in the investigated Landrace population (number of individuals genotyped n = 67), one in the Pietrain population (n = 40), 0.705 in the Large White population (n = 36), 0.885 in the Bazna population (n = 13), and 0.555 in the Mangalita population (n = 11). For all breeds, except Pietrain (monomorphic), the genotype distribution was in accordance with the Hardy&ndash
Weinberg equilibrium. Given that &beta
casein is the most important protein in sows&rsquo
milk, a polymorphism like the one described here may prove interesting for marker-assisted selection.
Databáze: OpenAIRE
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