Mathematical basis for the optimization of single cell-line screening on multi-well plates
| Autor: | Dengbo Ma, James W. Wells |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cell Survival
Cell Culture Techniques Colony Count Microbial Bioengineering CHO Cells Cell Separation Biology Transfection Models Biological Applied Microbiology and Biotechnology Cricetulus Cricetinae Muscarinic cholinergic Animals Computer Simulation Receptor Muscarinic M2 Models Statistical Basis (linear algebra) Chinese hamster ovary cell fungi General Medicine Stable transfection Molecular biology Recombinant Proteins Cell culture Sample Size Biological system Algorithms Statistical Distributions Biotechnology |
| Zdroj: | Biotechnology Letters. 26:1441-1446 |
| ISSN: | 0141-5492 |
| Popis: | Screening of stably transfected cells on multi-well plates is most efficient when a maximum number of wells contain a single colony. The multinomial distribution has been used to derive a novel equation that relates the number of wells containing a specified number of cell-lines and the total number of viable cells loaded on the plate. To test its validity, Chinese hamster ovary cells were transfected with a gene coding for the M2 muscarinic cholinergic receptor and screened on 96-well plates. The observed and predicted numbers of wells containing a single cell-line were indistinguishable. This approach therefore can be used to optimize the conditions for screening transfected cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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