Cross-species amplification and characterization of new microsatellite markers for the macaw palm,Acrocomia aculeata(Arecaceae)
| Autor: | Fekadu Gebretensay Mengistu, Cosme Damião Cruz, Kacilda Naomi Kuki, Eveline Teixeira Caixeta, Sérgio Yoshimitsu Motoike |
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| Přispěvatelé: | Fekadu Gebretensay Mengistu, Universidade Federal de Viçosa, Departamento de Fitotecnia, Sérgio Yoshimitsu Motoike, Universidade Federal de Viçosa, Departamento de Fitotecnia, EVELINE TEIXEIRA CAIXETA MOURA, CNPCa, Cosme Damião Cruz, Universidade Federal de Viçosa, Departamento de Biologia Geral. |
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germplasm Macauba Macaúba Population genetics Plant Science Arecaceae 01 natural sciences Domestication 03 medical and health sciences Elaeis oleifera Genetic variation Genetics biology Acrocomia aculeata Null alleles biology.organism_classification Transferability SSRs 030104 developmental biology Genetic marker Evolutionary biology Microsatellite Biodiesel Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| ISSN: | 1479-263X 1479-2621 |
| DOI: | 10.1017/s1479262115000179 |
| Popis: | Microsatellites or simple sequence repeats (SSRs) are useful molecular markers allowing for efficient conservation and sustainable use of genetic resources of plant species. Development of SSR marker system for new species is a very expensive task and time consuming. Cross-species amplification of microsatellite loci is considered as a cost-effective approach for developing microsatellite markers for new species. The aim of this work was to examine the transferability of some SSR markers of twoArecaceaespecies (Astrocaryum aculeatumandElaeis oleifera), inAcrocomia aculeata. Out of the total markers analysed 44% of the markers successfully amplified the genomic DNA inA. aculeata, of which 26% were polymorphic detecting a range of three to eight alleles with an average of 4.5 per locus. High average percentage of polymorphic loci (P= 71.2%) per provenance was obtained within a range of 57–100% detecting genetic variation inA. aculeatagermplasm collections. The polymorphic markers detected a positive inbreeding coefficient (F>0) per locus revealing heterozygote deficiency in the accessions that were analysed. As the cross-amplification was at family level, in which the taxonomic distance is relatively wider between the sources (A. aculeatumandE. oleifera) and the target (A. aculeata) species, the amplification success was relatively low. However, the results are promising and implicated that high cross-amplification success could be achieved at species or genus level inA. aculeata. The markers will contribute towards the domestication of the potential macaw palm through realizing various studies such as population genetics, germplasm characterization, genetic improvement and conservation. |
| Databáze: | OpenAIRE |
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