A second European collaborative study on polymerase chain reaction for Toxoplasma gondii, involving 15 teams
| Autor: | Maria Cristina Angelici, Hervé Pelloux, Dominique Krüger, Rosemarie Blatz, Edward Guy, Maija Lappalainen, Mona Holberg-Petersen, Regina Gratzl, Veronique Girault, Marie-Hélène Bessières, Mats Olsson, Horst Aspöck, Mariassunta Del Pezzo, Anne Naessens, J. D. Johnson |
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| Rok vydání: | 1998 |
| Předmět: |
Quality Control
European community 030231 tropical medicine Biology Rh strain Microbiology Polymerase Chain Reaction Toxoplasmosis Congenital law.invention 03 medical and health sciences 0302 clinical medicine law Pregnancy Prenatal Diagnosis Genetics medicine media_common.cataloged_instance Animals Humans False Positive Reactions European Union European union Molecular Biology False Negative Reactions Polymerase chain reaction media_common 0303 health sciences 030306 microbiology Infant Newborn Toxoplasma gondii DNA Protozoan biology.organism_classification medicine.disease Amniotic Fluid Virology Congenital toxoplasmosis Toxoplasmosis 3. Good health Evaluation Studies as Topic Pregnancy Complications Parasitic Immunology Female Laboratories Toxoplasma |
| Zdroj: | FEMS microbiology letters. 165(2) |
| ISSN: | 0378-1097 |
| Popis: | In order to investigate the accuracy and practicability of the polymerase chain reaction (PCR) in the antenatal diagnosis of congenital toxoplasmosis, a collaborative study involving 15 European laboratories was performed under the auspices of the Biomed 2 Programme of the European Community. Each team received 12 aliquots (four negative, eight positive) of 'artificial samples' made of amniotic fluid spiked with tachyzoites of the RH strain of Toxoplasma gondii. Each team performed its own PCR protocol (all were different). Nine of the 15 laboratories were able to detect a single parasite, but two of the 15 found all samples negative. Four of the 15 laboratories found one or more control samples to be falsely positive. This study highlights the lack of homogeneity between PCR protocols and performance and underlines the need for an external quality assurance scheme which could provide 'reference' samples that could be used by any laboratory wanting to establish and maintain an accurate diagnostic test based on PCR. |
| Databáze: | OpenAIRE |
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