Presence of the house dust mite allergen in the gastrointestinal tract of dogs with chronic enteropathy: A potential inducer of interleukin-1β
| Autor: | Masaki Miyajima, Kaho Takahashi, Hironari Osada, Nanako Yanuma, Misato Ogawa, Marin Hirokawa, Koji Sugita, Keitaro Ohmori, Hirotaka Kondo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
040301 veterinary sciences Immunology Interleukin-1beta Immunologic Tests medicine.disease_cause Allergic inflammation Arthropod Proteins 0403 veterinary science Pathogenesis 03 medical and health sciences Allergen Dogs Intestinal mucosa medicine Animals Intestinal Mucosa Feces 030304 developmental biology House dust mite 0303 health sciences Gastrointestinal tract General Veterinary biology Macrophages Pyroglyphidae Interleukin 04 agricultural and veterinary sciences Allergens biology.organism_classification Gastrointestinal Tract Intestinal Diseases Chronic Disease Female |
| Zdroj: | Veterinary immunology and immunopathology. 230 |
| ISSN: | 1873-2534 |
| Popis: | House dust mite (HDM) is an environmental allergen ubiquitously present indoors, causing allergic inflammation in dogs. However, it is unclear whether HDM allergens can be detected in the gastrointestinal (GI) tract of dogs. In addition, although expression of interleukin (IL)-1β is increased in the intestinal mucosa of dogs with chronic enteropathy (CE), the role of HDM allergens in the production of IL-1β has not been evaluated. The objectives of this study were to determine the presence of HDM allergens in the GI tract of dogs and to elucidate the effect of HDM on IL-1β expression in canine macrophages. HDM allergen, Dermatophagoides pteronyssinus (Der p) 1, was quantified in the gastric and duodenal fluids and the duodenal and colonic mucosae of dogs with CE and healthy laboratory dogs, and faeces of dogs with CE, healthy laboratory dogs and healthy client-owned dogs. Gene expression and protein levels of IL-1β were measured in HDM-stimulated canine peripheral macrophages from healthy laboratory dogs. Der p 1 was detected in the gastric and duodenal fluids of dogs with CE and healthy laboratory dogs, and faeces of all dogs examined. Der p 1 levels in the duodenal and colonic mucosae were significantly higher in dogs with CE than in healthy laboratory dogs. HDM increased both gene expression and protein levels of IL-1β in canine macrophages. These findings demonstrate the presence of HDM allergens in the GI tract of dogs and the possible involvement of HDM allergens in the pathogenesis of CE by promoting IL-1β expression in macrophages. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect