Molecular Properties of Water-Unextractable Proteoglycans from Hypsizygus marmoreus and Their in Vitro Immunomodulatory Activities
| Autor: | SangGuan You, Hee Mun Chae, Hong Hui Bao, Mehdi Tarbasa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Stereochemistry
Pharmaceutical Science immunomodulation activity Article Cell Line Analytical Chemistry lcsh:QD241-441 Mice chemistry.chemical_compound lcsh:Organic chemistry Polysaccharides Drug Discovery Mole Animals Immunologic Factors Monosaccharide Physical and Theoretical Chemistry chemistry.chemical_classification proteoglycan Molecular mass biology Macrophages Organic Chemistry Water Hypsizygus marmoreus Glycosidic bond In vitro Molecular Weight carbohydrates (lipids) Solubility molecular conformation chemistry Biochemistry Proteoglycan Chemistry (miscellaneous) Cell culture Galactose biology.protein Cytokines Molecular Medicine Proteoglycans glycosidic linkages Agaricales |
| Zdroj: | Molecules; Volume 17; Issue 1; Pages: 207-226 Molecules, Vol 17, Iss 1, Pp 207-226 (2011) Molecules |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules17010207 |
| Popis: | Four proteoglycans were sequentially extracted from Hypsizygus marmoreus using 0.1 M NaOH (alkali-soluble proteoglycans [F1] and alkali-insoluble proteoglycans [F3]) and 0.1 M HCl (acid-soluble proteoglycans [F2] and acid-insoluble proteoglycans [F4]), and their structures and immunomodulatory activities were investigated. The proteoglycans were found to contain carbohydrates (19.8-82.4%) with various amounts of proteins (7.7-67.3%), and glucose was the major monosaccharide unit present, along with trace amounts of galactose. The molecular weights (Mw) and the radius of gyration (Rg) of these proteoglycans showed ranges of 16 × 10(4)-19,545 × 10(4) g/mol and 35-148 nm, respectively, showing significant variations in their molecular conformations. The backbones of F1 and F2 were mainly connected through a-(1→3), (1→4) and b-(1→6)-glycosidic linkages with some branches. The F1 and F2 proteoglycans significantly stimulated Raw264.7 cells to release nitric oxide (NO), prostaglandin E2 (PGE(2)) and various cytokines, such as IL-1β, TNF-α and IL-6 by inducing their mRNA expressions. |
| Databáze: | OpenAIRE |
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