Induction of varicella zoster virus DNA replication in dissociated human trigeminal ganglia

Autor: Hussain Badani, Donald H. Gilden, Randall J. Cohrs, Teresa White, Bridget Sanford, Nicholas L. Baird
Rok vydání: 2016
Předmět:
Adult
DNA Replication
Male
0301 basic medicine
Herpesvirus 3
Human
viruses
Biology
Virus Replication
medicine.disease_cause
Herpes Zoster
Article
Tissue Culture Techniques
Phosphatidylinositol 3-Kinases
03 medical and health sciences
Cellular and Molecular Neuroscience
Tissue culture
Trigeminal ganglion
0302 clinical medicine
Virology
Nerve Growth Factor
Virus latency
medicine
Humans
Protein Kinase Inhibitors
Aged
Phosphoinositide-3 Kinase Inhibitors
integumentary system
Virus Activation
Varicella zoster virus
DNA replication
virus diseases
Middle Aged
medicine.disease
Antibodies
Neutralizing
Virus Latency
030104 developmental biology
Nerve growth factor
Gene Expression Regulation
Trigeminal Ganglion
Neurology
Viral replication
DNA
Viral
Host-Pathogen Interactions
Autopsy
Neurology (clinical)
030217 neurology & neurosurgery
Signal Transduction
Zdroj: Journal of NeuroVirology. 23:152-157
ISSN: 1538-2443
1355-0284
DOI: 10.1007/s13365-016-0480-1
Popis: Varicella zoster virus (VZV), a human neurotropic alphaherpesvirus, becomes latent after primary infection and reactivates to produce zoster. To study VZV latency and reactivation, human trigeminal ganglia removed within 24 h after death were mechanically dissociated, randomly distributed into six-well tissue culture plates and incubated with reagents to inactivate nerve growth factor (NGF) or phosphoinositide 3-kinase (PI3-kinase) pathways. At 5 days, VZV DNA increased in control and PI3-kinase inhibitor-treated cultures to the same extent, but was significantly more abundant in anti-NGF-treated cultures (p = 0.001). Overall, VZV DNA replication is regulated in part by an NGF pathway that is PI3-kinase-independent.
Databáze: OpenAIRE
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