Downregulation of Umbilical Cord Blood Levels of miR-374a in Neonatal Hypoxic Ischemic Encephalopathy
| Autor: | Gerard W. O'Keeffe, Deirdre M. Murray, Ailis Fagan, Gerard Clarke, John F. Cryan, Ted Dinan, Geraldine B. Boylan, Brian H. Walsh, Gerard M. Moloney, AM Looney, Sue Grenham |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Pathology Encephalopathy Down-Regulation Real-Time Polymerase Chain Reaction Gastroenterology Umbilical cord Severity of Illness Index Hypoxic Ischemic Encephalopathy Pregnancy Internal medicine Medicine Humans Prospective Studies Prospective cohort study Asphyxia Neonatorum business.industry Infant Newborn Infant Electroencephalography medicine.disease Fetal Blood Microarray Analysis Perinatal asphyxia MicroRNAs Real-time polymerase chain reaction medicine.anatomical_structure Cord blood Pediatrics Perinatology and Child Health Hypoxia-Ischemia Brain Female business |
| Zdroj: | The Journal of pediatrics. 167(2) |
| ISSN: | 1097-6833 |
| Popis: | Objective To investigate the expression profile of microRNA (miRNA) in umbilical cord blood from infants with hypoxic ischemic encephalopathy (HIE). Study design Full-term infants with perinatal asphyxia were identified under strict enrollment criteria. Degree of encephalopathy was defined using both continuous multichannel electroencephalogram in the first 24 hours of life and modified Sarnat score. Seventy infants (18 controls, 33 with perinatal asphyxia without HIE, and 19 infants with HIE [further graded as 13 mild, 2 moderate, and 4 severe]) were included in the study. MiRNA expression profiles were determined using a microarray assay and confirmed using quantitative real-time polymerase chain reaction. Results Seventy miRNAs were differentially expressed between case and control groups. Of these hsa-miR-374a was the most significantly downregulated in infants with HIE vs controls. Validation of hsa-miR-374a expression using quantitative real-time polymerase chain reaction confirmed a significant reduction in expression among infants with HIE compared with those with perinatal asphyxia and healthy controls (mean relative quantification [SD] = 0.52 [0.37] vs 1.10 [1.52] vs 1.76 [1.69], P Conclusions We have shown a significant step-wise downregulation of hsa-miR-374a expression in cord blood of infants with perinatal asphyxia and subsequent HIE. |
| Databáze: | OpenAIRE |
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