Use of Polyphosphates and Soluble Pyrophosphatase Activity in the Seaweed Ulva pseudorotundata
| Autor: | Juan J. Vergara, José Lucas Pérez-Lloréns, Patricia Herrera-Pérez, Fernando G. Brun |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
pyrophosphate
0106 biological sciences Pyrophosphatase Ulva 010604 marine biology & hydrobiology Polyphosphate Phosphorus pyrophosphatase activity chemistry.chemical_element Phosphate 010603 evolutionary biology 01 natural sciences Pyrophosphate chemistry.chemical_compound polyphosphates chemistry Dry weight seaweed Chlorophyll Ammonium Food science phosphorus |
| Zdroj: | Oceans Volume 1 Issue 4 Pages 23-354 |
| ISSN: | 2673-1924 |
| DOI: | 10.3390/oceans1040023 |
| Popis: | The hydrolytic activity of different types of polyphosphates, and the induction of soluble pyrophosphatase (sPPase EC 3.6.1.1) activity have been assessed in cell extracts of nutrient limited green seaweed Ulva pseudorotundata Cormaci, Furnari & Alongi subjected to different phosphorus regimes. Following a long period of nutrient limitation, the addition of different types of (poly)phosphates to artificial seawater enhanced growth rates on fresh weight and area, but not on dry weight bases. Chlorophyll and internal P content were affected by P supply. In contrast, internal soluble reactive P was kept low and was little affected by P additions. Soluble protein content increased in all treatments, as ammonium was added to prevent N limitation. The C:N:P atomic ratio revealed great changes depending on the nutrient regime along the experiment. Cell extracts of U. pseudorotundata were capable of hydrolyzing polyphosphates of different chain lengths (pyro, tripoly, trimeta, and polyphosphates) at high rates. The sPPase activity was kept very low in P limited plants. Following N and different kind of P additions, sPPase activity was kept low in the control, but slightly stimulated after 3 days when expressed on a protein basis. The highest activities were found at the end of the experiment under pyro and polyphosphate additions (7 days). The importance of alternative P sources to phosphate and the potential role of internal soluble pyrophosphatases in macroalgae are discussed. |
| Databáze: | OpenAIRE |
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