Rapid determination of total benzalkonium chloride content in ophthalmic formulation
| Autor: | Suzanne N. Dumont, Suzanne Levesque, Louis-Philippe Labranche, Alain Carrier |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Chemistry
Pharmaceutical Clinical Biochemistry Pharmaceutical Science Excipient Diluent High-performance liquid chromatography Analytical Chemistry Benzalkonium chloride Simple sample Drug Discovery medicine Chromatography High Pressure Liquid Spectroscopy Chromatography Chemistry Reversed-phase chromatography Reference Standards Solutions Anti-Infective Agents Local Indicators and Reagents Spectrophotometry Ultraviolet Particle size Ophthalmic Solutions Benzalkonium Compounds Quantitative analysis (chemistry) medicine.drug |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 43:989-993 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2006.09.022 |
| Popis: | A simple and rapid reversed-phase HPLC method was developed for routine analysis of total benzalkonium chloride in ophthalmic formulations. The analysis involves simple sample preparation using the mobile phase as the diluent. The method uses a Waters SymmetryShield RP-18 (75 mm × 4.6 mm, 3.5 μm particle size) column and a mobile phase consisting of a mixture of methanol–potassium phosphate (pH 3.0; 7.5 mM) (68:32, v/v). Using these conditions, three major homologs of the benzalkonium chloride (C 12 , C 14 and C 16 ) were separated in less then 7 min. Furthermore, recoveries ranging from 97% to 99% at three levels of the label claim of total benzalkonium chloride content were obtained for different ophthalmic formulations. Data supporting the development and validation of this method are presented. |
| Databáze: | OpenAIRE |
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