Altered blood rheology and impaired pressure-induced cutaneous vasodilation in a mouse model of combined type 2 diabetes and sickle cell trait

Autor: Kiao Ling Liu, Camille Romanet-Faes, Elie Nader, Cyril Martin, Dominique Sigaudo-Roussel, Mor Diaw, Emmanuelle Charrin, Philippe Connes, Bérengère Fromy, Audrey Josset-Lamaugarny, Sarah Skinner, Ming Lo, Vincent Pialoux
Přispěvatelé: Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire de Biologie Tissulaire et d'ingénierie Thérapeutique UMR 5305 (LBTI), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physiologie, Faculté de Médecine et de Pharmacie, Institut de biologie et chimie des protéines [Lyon] (IBCP), Fiabilité / Puissance, Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physiologie-Biologie du Sport, Université d'Auvergne - Clermont-Ferrand I (UdA)
Rok vydání: 2018
Předmět:
0301 basic medicine
Genetically modified mouse
Blood Glucose
medicine.medical_specialty
endocrine system diseases
[SDV]Life Sciences [q-bio]
Blood viscosity
Vasodilation
Blood Pressure
Mice
Transgenic
Type 2 diabetes
030204 cardiovascular system & hematology
Hematocrit
Biochemistry
Diabetes Mellitus
Experimental
Sickle Cell Trait
[SCCO]Cognitive science
03 medical and health sciences
0302 clinical medicine
hemic and lymphatic diseases
Internal medicine
Erythrocyte Deformability
medicine
Erythrocyte deformability
Animals
ComputingMilieux_MISCELLANEOUS
Skin
Sickle cell trait
medicine.diagnostic_test
business.industry
nutritional and metabolic diseases
Cell Biology
medicine.disease
Blood Viscosity
3. Good health
surgical procedures
operative
030104 developmental biology
Endocrinology
Hemorheology
Sodium nitroprusside
Cardiology and Cardiovascular Medicine
business
human activities
Diabetic Angiopathies
medicine.drug
Zdroj: Microvascular Research
Microvascular Research, Elsevier, 2019, 122, pp.111-116. ⟨10.1016/j.mvr.2018.11.014⟩
ISSN: 1095-9319
0026-2862
DOI: 10.1016/j.mvr.2018.11.014⟩
Popis: Objective Type 2 diabetes (T2D)-related vascular dysfunction and hemorheological abnormalities could possibly be amplified by sickle cell trait (SCT). These alterations could potentially increase the risk of vascular complications in individuals with combined T2D and SCT. Therefore, this study used a mouse model to determine whether vascular function and blood rheology were more severely altered in combined T2D and SCT than in T2D or SCT alone. Methods Townes transgenic mice with or without SCT received a 12-week high fat high sucrose or standard diet to create models of combined T2D-SCT, T2D, SCT, and controls. Pressure-induced vasodilation (PIV) and sodium nitroprusside (SNP)-mediated vasodilation in-vivo, and hemorheological parameters were measured. Results No significant differences in blood viscosity, hematocrit, erythrocyte deformability, or PIV were observed between the control and T2D mice, or the control and SCT mice. However, blood viscosity, erythrocyte deformability, and PIV were significantly altered in the T2D-SCT mice compared to the control mice. There were no differences in SNP response between the groups. Conclusions Although neither T2D nor SCT alone had significant effects on blood rheology parameters or vascular function, combined T2D-SCT mice had significantly altered blood rheology and significantly impaired vascular function.
Databáze: OpenAIRE
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