Molecular cloning and gene expression of Cg-Foxl2 during the development and the adult gametogenetic cycle in the oyster Crassostrea gigas

Autor: Blandine Diss, Marie-Laure Specq, Anne-Sophie Martinez, Michel Mathieu, Amine Naimi, Pascal Sourdaine
Přispěvatelé: Physiologie et Ecophysiologie des Mollusques Marins (PE2M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), SATMAR
Jazyk: angličtina
Rok vydání: 2009
Předmět:
0106 biological sciences
Male
Physiology
Cellular differentiation
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
Lophotrochozoa
Development
010603 evolutionary biology
01 natural sciences
Biochemistry
Gametogenesis
03 medical and health sciences
Complementary DNA
medicine
Animals
Humans
Amino Acid Sequence
Cloning
Molecular
Crassostrea
Molecular Biology
Phylogeny
030304 developmental biology
Regulation of gene expression
0303 health sciences
biology
Oyster
Ovary
Adult gametogenetic cycle
Gene Expression Regulation
Developmental
Forkhead Transcription Factors
Sequence Analysis
DNA
Gonad
Pacific oyster
Sex Determination Processes
Sex determination
biology.organism_classification
Molecular biology
Cg-DMl
Foxl2
medicine.anatomical_structure
Crassostrea gigas
Female
Vitellogenesis
Germ cell
Biomarkers
Zdroj: Comparative Biochemistry and Physiology-Part B: Biochemistry and Molecular Biology
Comparative Biochemistry and Physiology-Part B: Biochemistry and Molecular Biology, Elsevier, 2009, 154 (1), pp.134-142. ⟨10.1016/j.cbpb.2009.05.011⟩
ISSN: 1096-4959
DOI: 10.1016/j.cbpb.2009.05.011⟩
Popis: International audience; A Foxl2 ortholog has been identified in a lophotrochozoa, the pacific oyster, which is a successive irregular hermaphrodite mollusc. Its cDNA has been called Cg-Foxl2 (Crassostrea gigas Foxl2) and the deduced protein sequence is 367aa long. This sequence contains the conserved domain Forkhead box and its gene is devoid of intron at least in the first 926 bp of the cDNA, as found for Foxl2 factors. Real time PCR and in situ hybridization have shown a gonadic male and female Cg-Foxl2 expression which increases during the adult gametogenetic cycle for both sexes, but with a significant increase occurring earlier in females than in males. In females this increase corresponds to the vitellogenetic stage. During development, a peak of Cg-DMl (a potential factor of the male gonadic differentiation) and Oyvlg (a germ cell marker) expression and a significant decrease of Cg-Foxl2 expression were observed after metamorphosis in 1–1.5-month-old spats, a period of development when primordial germ cells may differentiate into germinal stem cells during the first gonadic establishmen
Databáze: OpenAIRE
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