Effect of implantation site on outcome of tissue-engineered vascular grafts

Autor: Sarra de Valence, Jean-Christophe Tille, Damiano Mugnai, Jean Pierre Giliberto, Michael Moeller, Robert Gurny, Azhari Yakub, Jeswant Dillon, Beat H. Walpoth, Tornike Sologashvili, Zamrin Dimon, Shahrul A. Saat
Rok vydání: 2019
Předmět:
Abdominal/physiopathology/surgery
Intimal hyperplasia
Carotid Arteries/physiopathology/surgery
Pharmaceutical Science
02 engineering and technology
ddc:616.07
030226 pharmacology & pharmacy
Graft Occlusion
Rats
Sprague-Dawley
Lactones
0302 clinical medicine
Models
Occlusion
Medicine
Common carotid artery
Aorta
Abdominal
Aorta
Caproates/chemistry
ddc:615
medicine.diagnostic_test
ddc:617
Abdominal aorta
Graft Occlusion
Vascular
General Medicine
021001 nanoscience & nanotechnology
Cellular infiltration
Carotid Arteries
Lactones/chemistry
Models
Animal
cardiovascular system
0210 nano-technology
Blood Vessel Prosthesis Implantation/adverse effects/instrumentation/methods
Biotechnology
medicine.medical_specialty
Polyesters
03 medical and health sciences
Standard anatomical position
Blood Vessel Prosthesis Implantation
medicine.artery
Animals
Caproates
Vascular Patency
Polyesters/chemistry
business.industry
Animal
Blood Vessel Prosthesis/adverse effects
Vascular/diagnostic imaging/etiology/physiopathology
medicine.disease
Surgery
Blood Vessel Prosthesis
Rats
Angiography
Sprague-Dawley
business
Calcification
Zdroj: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 139 (2019) pp. 272-278
ISSN: 1873-3441
0939-6411
Popis: Objective Vascular prostheses for small caliber bypass grafts in cardiac and vascular diseases or for access surgery are still missing. Poly (Ɛ-caprolactone) (PCL) has been previously investigated by our group and showed good biocompatibility and mechanical properties in vitro and rapid endothelialisation, cellular infiltration and vascularisation in vivo yielding optimal patency in the abdominal aortic position. The aim of the present study is to evaluate our PCL graft in the carotid position and to compare its outcome to the grafts implanted in the abdominal aortic position. Methods PCL grafts (1 mm ID/10 mm long) were implanted into the left common carotid artery in 20 Sprague-Dawley rats and compared to our previously published series of abdominal aortic implants. The animals were followed up to 3, 6, 12 and 24 weeks. At each time point, in vivo compliance, angiography and histological examination with morphology were performed. Results PCL grafts showed good mechanical properties and ease of handling. The average graft compliance was 14.5 ± 1.7%/ mmHg compared to 7.8 ± 0.9% for the abdominal position and 45.1 ± 3.2%/ mmHg for the native carotid artery. The overall patency for the carotid position was 65% as compared to 100% in the abdominal position. Complete endothelialisation was achieved at 3 weeks and cell invasion was more rapid than in the aortic position. In contrast, intimal hyperplasia (IH) and vascular density were less pronounced than in the aortic position. Conclusion Our PCL grafts in the carotid position were well endothelialised with early cellular infiltration, higher compliance, lower IH and calcification compared to the similar grafts implanted in the aortic position. However, there was a higher occlusion rate compared to our abdominal aorta series. Anatomical position, compliance mismatch, flow conditions may answer the difference in patency seen.
Databáze: OpenAIRE
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