Comparison of the regenerative effect of adipose-derived stem cells, fibrin glue scaffold, and autologous bone graft in experimental mandibular defect in rabbit
| Autor: | Hanieh Shaterzadeh-Yazdi, Mahsa Omidi, B. Zamiri, Davood Mehrabani, Azizollah Khodakaram-Tafti |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_specialty Scaffold 040301 veterinary sciences Mandibular Osteotomy Adipose tissue Fibrin Tissue Adhesive Transplantation Autologous 0403 veterinary science Random Allocation 03 medical and health sciences 0302 clinical medicine otorhinolaryngologic diseases medicine Animals Fibrin glue Wound Healing Bone Transplantation Tissue Scaffolds business.industry Stem Cells Regeneration (biology) Mandible 030206 dentistry 04 agricultural and veterinary sciences Surgery Disease Models Animal surgical procedures operative medicine.anatomical_structure Adipose Tissue Orthopedic surgery Cortical bone Rabbits Oral Surgery Stem cell Tomography X-Ray Computed business Stem Cell Transplantation |
| Zdroj: | Dental Traumatology. 34:413-420 |
| ISSN: | 1600-4469 |
| DOI: | 10.1111/edt.12435 |
| Popis: | Background/aims One of the main concerns for maxillofacial and orthopedic surgeons is finding a method to improve regeneration of large craniofacial bone defects. The aim of this study was to investigate the healing and regenerative effects of fibrin glue associated with adipose-derived stem cells (ADSCs) and fibrin glue scaffold alone with autologous bone grafts in experimental mandibular defects of the rabbit. Methods Bilateral uni-cortical osteotomies were performed in the mandible of 20 male Dutch rabbits. The animals were randomly divided into 2 equal groups. In one group, the defect on the right side was treated by fibrin glue associated with ADSCs and the defect on the other side remained as the control. In another group, the defect on the right side was treated with fibrin glue and on the left side with autologous bone graft. After 28 and 56 days, five rabbits from each group were evaluated by computed tomography (CT) and histopathological examinations. Results Coronal CT showed a remarkable reconstruction of cortical bone in the fibrin glue associated with ADSCs group at 28 and 56 days post-surgery. Histopathologically, new cortical bony bridge formation was seen increasingly in the fibrin glue, fibrin glue associated with ADSCs, and autologous bone graft groups after 28 days. Statistical analysis of the thickness of new cortical bone in the treatment versus control groups showed a significant difference between fibrin glue alone and fibrin glue associated with ADSCs groups (P = 0.02). No significant difference was found between the fibrin glue associated with ADSCs and the autologous bone graft groups (P > 0.05). Conclusions The healing process had a significant increase in the thickness of new cortical bone when fibrin glue scaffold associated with ADSCs was used. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text