Radiolabeled GX1 Peptide for Tumor Angiogenesis Imaging

Autor: Erica Aparecida de Oliveira, Ana Funari, Angélica Bueno Barbezan, Bluma Linkowski Faintuch, Roselaine Campos Targino, Daniele Seo, Ana Maria Moro
Rok vydání: 2018
Předmět:
Zdroj: Applied Biochemistry and Biotechnology. 185:863-874
ISSN: 1559-0291
0273-2289
DOI: 10.1007/s12010-018-2700-z
Popis: Early and accurate detection of primary or metastatic tumors is of great value in staging, treatment management, and prognosis. Tumor angiogenesis plays an essential role in the growth, invasion, and metastatic spread of solid cancers, and so, is a promising approach for tumor imaging. The GX1 (CGNSNPKSC) peptide was identified by phage display library and has been investigated as a marker for human cancers. This study aims to evaluate the 99mTc-HYNIC-PEG4-c (GX1) as a biomarker for tumor imaging. Our results showed that GX1 specifically binds to tumor cells in vitro. SKMEL28 and MDA-MB231 cells achieved total binding peak at 60 min of incubation. For B16F10 and MKN45 cells, the total and specific binding were similar during all time points, while A549 cell line showed rapid cellular total uptake of the tracer at 30 min of incubation. Biodistribution showed low non-specific uptakes and rapid renal excretion. Melanoma tumors showed enhanced GX1 uptake in animal model at 60 min, and it was significantly blocked by cold peptide. The radiotracer showed tumor specificity, especially in melanomas that are highly vascularized tumors. In this sense, it should be considered in future studies, aiming to evaluate degree of angiogenesis, progression, and invasion of tumors.
Databáze: OpenAIRE
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