Proteome Analyses of Soil Bacteria Grown in the Presence of Potato Suberin, a Recalcitrant Biopolymer
| Autor: | Lauriane Giroux, Vicky Toussaint, Carole Beaulieu, Sylvain Lerat, Anne-Marie Simao-Beaunoir, Amadou Sidibé |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Proteome Population Soil Science Plant Science 03 medical and health sciences Biopolymers Suberin Botany lipase Myxococcus xanthus education Ecology Evolution Behavior and Systematics Soil Microbiology Solanum tuberosum chemistry.chemical_classification education.field_of_study Oscillatoria biology Strain (chemistry) Bacteria Fatty acid General Medicine Articles biology.organism_classification Lipid Metabolism Lipids Carbon Culture Media 030104 developmental biology chemistry Biochemistry bacterial succession β-oxidation metaproteomics Soil microbiology |
| Zdroj: | Microbes and Environments |
| ISSN: | 1347-4405 1342-6311 |
| Popis: | Suberin is a complex lipidic plant polymer found in various tissues including the potato periderm. The biological degradation of suberin is attributed to fungi. Soil samples from a potato field were used to inoculate a culture medium containing suberin as the carbon source, and a metaproteomic approach was used to identify bacteria that developed in the presence of suberin over a 60-d incubation period. The normalized spectral counts of predicted extracellular proteins produced by the soil bacterial community markedly decreased from day 5 to day 20 and then slowly increased, revealing a succession of bacteria. The population of fast-growing pseudomonads declined and was replaced by species with the ability to develop in the presence of suberin. The recalcitrance of suberin was demonstrated by the emergence of auxotrophic bacteria such as Oscillatoria on the last days of the assay. Nevertheless, two putative lipases from Rhodanobacter thiooxydans (I4WGM2) and Myxococcus xanthus (Q1CWS1) were detected in the culture supernatants, suggesting that at least some bacterial species degrade suberin. When grown in suberin-containing medium, R. thiooxydans strain LCS2 and M. xanthus strain DK 1622 both produced three lipases, including I4WGM2 and Q1CWS1. These strains also produced other proteins linked to lipid metabolism, including fatty acid and lipid transporters and β-oxidation enzymes, suggesting that they participate in the degradation of suberin. However, only the R. thiooxydans strain appeared to retrieve sufficient carbon and energy from this recalcitrant polymer in order to maintain its population over an extended period of time. |
| Databáze: | OpenAIRE |
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