Integrin α-3 ß-1's central role in breast cancer, melanoma and glioblastoma cell aggregation revealed by antibodies with blocking activity
| Autor: | Brian Berger, David R. Soll, Ryan J. Reis, Tiffany S. Nop, Deborah Wessels, Daniel F. Lusche, Michael R. Klemme, Rebecca A. Glover, Cristopher C. Forrest, Benjamin A. Soll |
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| Rok vydání: | 2019 |
| Předmět: |
Immunology
Normal tissue Breast Neoplasms 03 medical and health sciences 0302 clinical medicine Breast cancer Cell Movement Cell Line Tumor Report medicine Cell Adhesion Immunology and Allergy Humans Antibodies Blocking neoplasms Melanoma 030304 developmental biology Cell Aggregation 0303 health sciences Matrigel Glioblastoma cell biology Chemistry CD44 Integrin alpha3beta1 Antibodies Monoclonal Integrin α medicine.disease Drug Combinations Hyaluronan Receptors 030220 oncology & carcinogenesis biology.protein Cancer research Female Proteoglycans Collagen Laminin Antibody Glioblastoma |
| Zdroj: | mAbs. 11(4) |
| ISSN: | 1942-0870 |
| Popis: | Breast cancer, melanoma and glioblastoma cells undergo cell-mediated aggregation and aggregate coalescence in a transparent 3D Matrigel environment. Cells from normal tissue and non-tumorigenic cell lines do not exhibit these behaviors. Here, 266 monoclonal antibodies (mAbs) demonstrated to interact with a wide variety of membrane, secreted and matrix proteins, have been screened for their capacity to block these tumorigenic cell-specific behaviors in a 3D environment. Remarkably, only six of the 266 tested mAbs exhibited blocking activity, four targeting integrin ß-1, one targeting integrin α-3 and one targeting CD44. Colocalization of integrins ß-1 and α-3 in fixed cells and in live aggregates suggests that the integrin α-3 ß-1 dimer plays a central role in cancer cell aggregation in the 3D environment provided by Matrigel. Our results suggest that blocking by anti-integrin and anti-CD44 mAbs involves interference in cell-cell interactions. |
| Databáze: | OpenAIRE |
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