Evaluation of a commercial gene probe for identification of Legionella cultures
| Autor: | Bonnie B. Plikaytis, H W Wilkinson, Jacquelyn S. Sampson |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Genus Legionella biology Legionella Nucleic Acid Hybridization Diagnostic test Bacterial Infections bacterial infections and mycoses biology.organism_classification Virology respiratory tract diseases Microbiology Serology RNA Bacterial Nucleic acid thermodynamics Evaluation Studies as Topic RNA Ribosomal Nucleic acid Humans bacteria Gene probe Positive test Research Article |
| Zdroj: | Journal of Clinical Microbiology. 23:217-220 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Confirmation of a culture as Legionella when it is unreactive with available serologic reagents involves tests that are impractical in most clinical laboratories. A nucleic acid probe that hybridizes only to members of the genus Legionella was recently prepared for marketing by Gen-Probe, Inc., San Diego, Calif. We tested 215 Legionella strains, representing 22 species, and 84 non-Legionella strains, representing 17 bacterial genera, with the Gen-Probe kit. All but four Legionella strains (L. bozemanii, less than 2% of total) and no heterologous strains gave positive test results. We conclude that the Legionella gene probe is a valuable addition to existing diagnostic tests for Legionella organisms. |
| Databáze: | OpenAIRE |
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