Rifampicin Resistance Phenotyping ofBrucella melitensisbyrpoBGene Analysis in Clinical Isolates
| Autor: | Murat Sayan, Yaşar E, Ayşe Willke, Devrim Dündar, Sevil Erdenlig, Zeki Yumuk, Bilenoglu O |
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| Rok vydání: | 2008 |
| Předmět: |
Brucellaceae
Single-nucleotide polymorphism Microbial Sensitivity Tests Polymorphism Single Nucleotide Microbiology Drug Resistance Bacterial Brucella melitensis polycyclic compounds medicine Humans Pharmacology (medical) Typing Antibiotics Antitubercular Gene Antibacterial agent Pharmacology Dose-Response Relationship Drug biology bacterial infections and mycoses biology.organism_classification rpoB Phenotype Infectious Diseases Oncology Genes Bacterial Mutation Rifampin Rifampicin medicine.drug |
| Zdroj: | Journal of Chemotherapy. 20:431-435 |
| ISSN: | 1973-9478 1120-009X |
| DOI: | 10.1179/joc.2008.20.4.431 |
| Popis: | R Rifampicin resistance of Brucella melitensis by rpoB gene analysis has not yet been performed in Turkey, where brucellosis is endemic. In this study, we investigated the efficacy of E-test and single nucleotide polymorphism (SNP) analysis of the B. melitensis rpoB gene, for the detection of mutations conferring rifampicin resistance, by sequencing 21 human B. melitensis strains from the Southeast and Marmara regions of Turkey. On CLSI slow-growing bacteria standards, all isolates were sensitive to rifampicin except for 6 which showed intermediate resistance to rifampicin. MIC(50) and MIC(90)values were 1 microg/ml and 1.5 microg/ml respectively (range 0.50 -1.5 microg/ml). The rifampicin-resistant phenotype was investigated at Cd 154 (GTT/TTT), Cd 526 (GAC/TAC, GAC/AAC, GAC/GGC), Cd 536 (CAC/CTC, CAC/TAC), Cd 539 (CGC/AGC), Cd 541 (TCG/TTG) and Cd 574 (CCG/CTG) of the rpoB gene in B. melitensis 16M and B115 strains, and in clinical isolates. No missense mutations were found in any of the B. melitensis isolates, which indicates that all isolates were rifampicin-susceptible. In conclusion, SNP analysis was useful as a molecular tool for rifampin resistance testing. Although resistance to rifampicin was not detected in our strains of B. melitensis; the presence of strains with intermediate resistance to rifampicin indicates that susceptibility testing should be performed periodically. |
| Databáze: | OpenAIRE |
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