Brain endothelial HSP-70 stress response coincides with endothelial and pericyte death after brain trauma
| Autor: | Christian W. Kreipke, José A. Rafols, Foaz Kayali, Donald J. DeGracia |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Pathology medicine.medical_specialty Programmed cell death Time Factors Endothelium Traumatic brain injury Biology Immunofluorescence Rats Sprague-Dawley In Situ Nick-End Labeling medicine Animals HSP70 Heat-Shock Proteins Heat shock TUNEL assay Cell Death medicine.diagnostic_test Brain General Medicine medicine.disease Rats Endothelial stem cell Disease Models Animal medicine.anatomical_structure Neurology Brain Injuries Neurology (clinical) Pericyte Pericytes Heat-Shock Response |
| Zdroj: | Neurological Research. 29:356-361 |
| ISSN: | 1743-1328 0161-6412 |
| DOI: | 10.1179/016164107x204666 |
| Popis: | Objectives: Our objective was to characterize the heat shock response (HSR) in a model of traumatic brain injury (TBI) and to determine the association of HSR to cell death. Methods: We used immunofluorescent detection of HSP-70 to characterize HSR and TUNEL labeling to determine the pattern of cell death. Results: HSP-70 immunofluorescence revealed a steady increase from 4 to 48 hours following TBI, culminating in a ubiquitous expression with the capillary bed 48 hours post-TBI. TUNEL labeling revealed a small subset of endothelial cell death and a most robust staining of putative pericyte cell death. Discussion: Our results show that while injury causes a detectable stress response, cell death is not a direct consequence of the HSR. |
| Databáze: | OpenAIRE |
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