The PHIST protein GEXP02 targets the host cytoskeleton during sexual development of Plasmodium falciparum
| Autor: | Françoise Brand, Lara Pérez-Martínez, Falk Butter, Jan D. Warncke, Nicholas I Proellochs, Till S. Voss, Enja Kipfer, Hans-Peter Beck, Armin Passecker, Taco W. A. Kooij |
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| Rok vydání: | 2020 |
| Předmět: |
Immunology
Plasmodium falciparum lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] Protozoan Proteins Microbiology Plasmodium Host-Parasite Interactions 03 medical and health sciences Virology Host cell periphery Gametocyte Parasite hosting Humans Malaria Falciparum 030304 developmental biology 0303 health sciences biology 030306 microbiology Erythrocyte Membrane biology.organism_classification Subtelomere Phenotype 3. Good health Cell biology Germ Cells Host cytoskeleton |
| Zdroj: | Cellular Microbiology, 22 Cellular Microbiology, 22, 2 |
| ISSN: | 1462-5814 |
| DOI: | 10.1111/cmi.13123 |
| Popis: | Contains fulltext : 218917pub.pdf (Publisher’s version ) (Closed access) A hallmark of the biology of Plasmodium falciparum blood stage parasites is their extensive host cell remodelling, facilitated by parasite proteins that are exported into the erythrocyte. Although this area has received extensive attention, only a few exported parasite proteins have been analysed in detail, and much of this remodelling process remains unknown, particularly for gametocyte development. Recent advances to induce high rates of sexual commitment enable the production of large numbers of gametocytes. We used this approach to study the Plasmodium helical interspersed subtelomeric (PHIST) protein GEXP02, which is expressed during sexual development. We show by immunofluorescence that GEXP02 is exported to the gametocyte-infected host cell periphery. Co-immunoprecipitation revealed potential interactions between GEXP02 and components of the erythrocyte cytoskeleton as well as other exported parasite proteins. This indicates that GEXP02 targets the erythrocyte cytoskeleton and is likely involved in its remodelling. GEXP02 knock-out parasites show no obvious phenotype during gametocyte maturation, transmission through mosquitoes, and hepatocyte infection, suggesting auxiliary or redundant functions for this protein. In summary, we performed a detailed cellular and biochemical analysis of a sexual stage-specific exported parasite protein using a novel experimental approach that is broadly applicable to study the biology of P. falciparum gametocytes. |
| Databáze: | OpenAIRE |
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