Antimicrobial Activity of Different Antimicrobial Peptides (AMPs) Against Clinical Methicillin-resistant Staphylococcus aureus (MRSA)
| Autor: | Wojciech Kamysz, Gianluca Morroni, Wally Baffone, D. Arzeni, Eleonora Ciandrini, Oscar Cirioni, Elżbieta Kamysz, Damian Neubauer, Raffaella Campana, Lucia Brescini |
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| Rok vydání: | 2019 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Dose-Response Relationship Drug Antimicrobial peptides Microbial Sensitivity Tests General Medicine Bacterial growth medicine.disease_cause Antimicrobial 01 natural sciences Methicillin-resistant Staphylococcus aureus Temporin Anti-Bacterial Agents 0104 chemical sciences Microbiology Structure-Activity Relationship 010404 medicinal & biomolecular chemistry chemistry.chemical_compound Minimum inhibitory concentration chemistry Staphylococcus aureus Drug Discovery medicine Growth inhibition Antimicrobial Cationic Peptides |
| Zdroj: | Current Topics in Medicinal Chemistry. 18:2116-2126 |
| ISSN: | 1568-0266 |
| DOI: | 10.2174/1568026618666181022140348 |
| Popis: | Background: Antimicrobial research is being focused to look for more effective therapeutics against antibiotic-resistant infections caused by methicillin-resistant Staphylococcus aureus (MRSA). In this direction, antimicrobial peptides (AMP) appear as promising tool. Objectives: This study evaluated the antimicrobial activity of different AMPs (Citropin 1.1, Temporin A, Pexiganan, CA(1–7)M(2–9)NH2, Pal-KGK-NH2, Pal-KKKK-NH2, LL-37) against human MRSA clinical isolates. Methods: The Minimum Inhibitory Concentration (MIC) was assessed for each AMP; then, the most active ones (Citropin 1.1, Temporin A, CA(1–7)M(2–9)NH2 and Pal-KGK-NH2) were tested against selected MRSA strains by time-kill studies. Results: The lowest MIC value was observed for Pal-KGK-NH2 (1 µg/ml), followed by Temporin A (4- 16 µg/ml), CA(1–7)M(2–9)NH2 (8-16 µg/ml) and Citropin 1.1 (16-64 µg/ml), while higher MICs were evidenced for LL-37, Pexiganan and Pal-KKKK-NH2 (> 128 µg/ml). In time-kill experiments, Citropin 1.1 and CA(1-7)M(2-9)NH2 showed a relatively high percentage of growth inhibition (>30 %) for all the tested MRSA clinical isolates, with a dose-dependent activity resulting in the highest percentage of bacterial growth inhibition (89.39%) at 2MIC concentration. Conclusion: Overall, our data demonstrated the potential of some AMPs against MRSA isolates, such as Citropin 1.1 and CA(1-7)M(2-9)NH2, that represents a promising area of development for different clinical applications. |
| Databáze: | OpenAIRE |
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