Evaluation of a modified culture medium for Borrelia burgdorferi sensu lato
| Autor: | Reto Lienhard, Islay Rodríguez, Fatima Jouda, Marie Colette Veuve, Hans H. Siegrist, Carmen Fernández, José Rodríguez, Lise Gern |
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| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
Time Factors BSK-H medium lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 new medium lcsh:QR1-502 Protein profile lcsh:Microbiology Microbiology Bacterial protein Bacterial Proteins Borrelia burgdorferi Group Sensu Borrelia Borrelia burgdorferi Sensu stricto biology Strain (chemistry) bacterial infections and mycoses biology.organism_classification proteins Culture Media Borrelia species |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 102, Iss 8, Pp 999-1002 (2007) Memórias do Instituto Oswaldo Cruz, Volume: 102, Issue: 8, Pages: 1002-999, Published: DEC 2007 |
| ISSN: | 0074-0276 |
| DOI: | 10.1590/s0074-02762007000800017 |
| Popis: | The aim of the present study was to assess the possible use of a modified medium, prepared in the laboratory using the constituents of Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the culture of Borrelia strains, comparing the growth of individual strains in this medium and in the BSK-H medium, and the protein profile and antigenic characteristics of Borrelia proteins expressed in these media. A qualitative evaluation of growth of Borrelia species was made with acceptable results (morphology and motility), but during a quantitative evaluation using the three main genospecies of Borrelia, the better results were obtained with a B. burgdorferi sensu stricto strain. The modified medium did not enable the growth of a B. afzelii strain. The protein profile and antigenic characteristic of the expressed proteins in the modified medium were studied with satisfactory results. These results suggest the modified medium as an alternative for the cultivation of Borrelia strains, with some limitations, in poorly-resourced laboratories. |
| Databáze: | OpenAIRE |
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