Correlation Analysis between Gut Microbiota and Metabolites in Children with Systemic Lupus Erythematosus
Autor: | Wei Xiang, Qiuyu Lin, Xiaojie He, Xuewei Ding, Min Wen, Xiaoyan Huang, Taohua Liu, Shipin Feng, Xiqiang Dang, Qing-Nan He, Zhiquan Xu, Xiaoyan Li, Mingyi Zhao |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Enterobacteriales Male Article Subject Protein digestion Immunology Disease Gut flora Gas Chromatography-Mass Spectrometry Pathogenesis 03 medical and health sciences Feces 0302 clinical medicine immune system diseases Metabolome medicine Immunology and Allergy Humans Lupus Erythematosus Systemic Metabolomics KEGG Child skin and connective tissue diseases 030203 arthritis & rheumatology biology Age Factors General Medicine RC581-607 biology.organism_classification medicine.disease Connective tissue disease Gastrointestinal Microbiome 030104 developmental biology Case-Control Studies Dysbiosis Female Disease Susceptibility Metagenomics Immunologic diseases. Allergy Biomarkers Immunosuppressive Agents Research Article |
Zdroj: | Journal of Immunology Research, Vol 2021 (2021) Journal of Immunology Research |
ISSN: | 2314-7156 2314-8861 |
Popis: | Systemic lupus erythematosus (SLE) is an autoimmune-mediated diffuse connective tissue disease characterized by immune inflammation with an unclear aetiology and pathogenesis. This work profiled the intestinal flora and faecal metabolome of patients with SLE using 16S RNA sequencing and gas chromatography-mass spectrometry (GC-MS). We identified unchanged alpha diversity and partially altered beta diversity of the intestinal flora. Another important finding was the increase in Proteobacteria and Enterobacteriales and the decrease in Ruminococcaceae among SLE patients. For metabolites, amino acids and short-chain fatty acids were enriched when long-chain fatty acids were downregulated in SLE faecal samples. KEGG analysis showed the significance of the protein digestion and absorption pathway, and association analysis revealed the key role of 3-phenylpropanoic acid and Sphingomonas. Sphingomonas were reported to be less abundant in healthy periodontal sites of SLE patients than in those of HCs, indicating transmission of oral species to the gut. This study contributes to the understanding of the pathogenesis of SLE disease from the perspective of intestinal microorganisms, explains the pathogenesis of SLE, and serves as a basis for exploring potential treatments for the disease. |
Databáze: | OpenAIRE |
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