An Overview on the Mechanisms and Applications of Enzyme Inhibition-Based Methods for Determination of Organophosphate and Carbamate Pesticides
| Autor: | He Yu, Miao Wang, A. M. Abd El-Aty, Jing Cao, Yongxin She, Ahmet Hacimuftuoglu, Jing Wang, Jiaming Ye, Shuibing Lao, Zhen Cao |
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| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Carbamate biology Artificial enzyme medicine.medical_treatment Organophosphate Biosensing Techniques General Chemistry Pesticide Acetylcholinesterase Abzyme chemistry.chemical_compound Organophosphorus Compounds Enzyme chemistry Biochemistry medicine biology.protein Carbamates Cholinesterase Inhibitors Enzyme Reactivation Pesticides General Agricultural and Biological Sciences Enzyme Assays |
| Zdroj: | Journal of Agricultural and Food Chemistry. 68:7298-7315 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Acetylcholinesterase inactivating compounds, such as organophosphate (OP) and carbamate (CM) pesticides, are widely used in agriculture to ensure sustainable production of food and feed. As a consequence of their applications, they would result in neurotoxicity, even death. In this essence, the development of enzyme inhibition methods still shows great significance as rapid detection techniques for on-site large-scale screening of OPs and CMs. Initially, mechanisms and applications of various enzyme-inhibition-based methods and devices, including optical colorimetric assay, fluorometric assays, electrochemical biosensors, rapid test card, and microfluidic device, are highlighted in the present overview. Further, to enhance the enzyme sensitivity for detection; alternative enzyme sources or high yield enrichment methods (such as abzyme, artificial enzyme, and recombinant enzyme), as well as enzyme reactivation and identification, are also addressed in this comprehensive overview. |
| Databáze: | OpenAIRE |
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