Isolation, characterization, and chromosomal location of a gene encoding theΔ1-pyrroline-5-carboxylate synthetase inArabidopsis thaliana

Autor: Wilson Ardiles, Arnould Savouré, Samir Jaoua, Xuejun Hua, Nathalie Verbruggen, Marc Van Montagu
Rok vydání: 1995
Předmět:
DNA
Complementary
Transcription
Genetic
Arabidopsis thaliana
Proline
Molecular Sequence Data
Salt stress
Saccharomyces cerevisiae
Arabidopsis
Biophysics
Sodium Chloride
Reductase
Biology
Genes
Plant
Polymerase Chain Reaction
Biochemistry
Protein Structure
Secondary
Homology (biology)
Exon
Gene Expression Regulation
Plant
Pyrroline-5-carboxylate synthetase
Structural Biology
Complementary DNA
Genetics
Amino Acid Sequence
Cloning
Molecular
Molecular Biology
Gene
DNA Primers
chemistry.chemical_classification
Leucine Zippers
Oxidoreductases Acting on CH-NH Group Donors
Base Sequence
Chromosome Mapping
Cell Biology
Phosphotransferases (Carboxyl Group Acceptor)
biology.organism_classification
Aldehyde Oxidoreductases
Molecular biology
Amino acid
1-Pyrroline-5-Carboxylate Dehydrogenase
Blotting
Southern
chemistry
Glutamate-5-Semialdehyde Dehydrogenase
NAD+ kinase
Sequence Alignment
Zdroj: FEBS Letters. 372:13-19
ISSN: 0014-5793
DOI: 10.1016/0014-5793(95)00935-3
Popis: A full-length cDNA and the corresponding At-P5S gene encoding the first enzyme of the proline biosynthetic pathway, the Δ 1 -pyrroline-5-carboxylate (P5C) synthetase, were isolated in Arabidopsis thaliana . The At-P5S cDNA encodes a protein of 717 amino acids showing high identity with the P5C synthetase of Vigna aconitifolia . Strong homology is also found at the N-terminus to bacterial and yeast γ-glutamyl kinase and at the C-terminus to bacterial γ-glutamyl phosphate reductase. Putative ATP- and NAD(P)H-binding sites are suggested in the At-P5S protein. The transcribed region of the At-P5S gene is 4.8 kb long and contains 20 exons. Southern analysis suggests the presence of only one At-P5S gene in the A. thaliana genome mapped at the bottom of the chromosome two. Expression analysis of At-P5S in different organs reveals abundant At-P5S transcripts in mature flowering plant. Rapid induction of the At-P5S gene followed by accumulation of proline was observed in NaCl-treated seedlings suggesting that At-P5S is osmoregulated.
Databáze: OpenAIRE
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